锥栗农家品种的遗传多样性及亲缘关系分析

采用ISSR技术对37个锥栗农家品种进行遗传多样性及亲缘关系分析。从65条通用引物中筛选出13条扩增效果较好的引物进行扩增,每条引物的扩增谱带从9(引物828) 16条(引物821)不等,平均每个引物产生12个ISSR片段,共扩增出156条谱带,其中多态性条带129条,占82.69%,平均每个引物扩增的DNA多态性条带为9.9条。结果表明:锥栗农家品种具有丰富的遗传多样性水平;供试农家品种的观测等位基因数1.826 9,有效等位基因数1.509 7,Nei's基因多样度(H_e)为0.292 3,Shannon信息指数为 0.434 4;13条引物可区分37份农家品种及其优株。根据遗传一致度构建反映品种间亲缘关系的UPGMA聚类图,37个锥栗品种可划分为2大类群7个亚类。

Analysis of Genetic Diversity and Genetic Relationships of Castanea henryi Native Varieties

ISSR was used to study the genetic diversity and genetic relationship of 37 Castanea henryi native varieties. 13 ISSR primers were selected from 65 universal primers. The number of bands produced by each primer varied from 9 to 16, the average was 12. A total of 156 bands were produced, in which 129 were polymorphic, the percentage of polymorphic loci was 82.69%, in average 9.9 polymorphic bands produced by each primer,and a relatively high level of genetic diversity was revealed by the result. The Observed number of alleles was 1.826 9, the effective number of alleles was 1.509 7, Nei's gene diversity was 0.292 3, Shannon information index was 0.434 4. 37 cultivars were distinguished by 13 ISSR primers. A dendrogram showed that the genetic relationship was constructed through an unweighted pair group method (UPGMA) based on the genetic identity and 37 cultivars were clustered into 2 main groups and 7 subgroups.