基于柑橘及其近缘属植物DNA条形码的叶绿体编码序列筛选

 【目的】通过对柑橘及其近缘属植物叶绿体4种编码序列的测定分析,获得能进行DNA条形编码的特征序列,为进一步研究叶绿体非编码区序列奠定基础。【方法】对柑橘及其近缘属植物59份样品进行matK、rpoB、rpoC1、rbcL测序,序列比对与人工校正,计算属间、种间、种内的遗传距离,比较序列间的差异,建立系统发育树。【结果】4种序列中,matK序列在属间、种间差异最大,与其它序列相比具有显著性差异,rbcL序列次之,而rpoB、rpoC1序列两者间没有显著性差异。【结论】matK序列是柑橘及其近缘属植物DNA条形码的未来研究中一个重要的候选片段。

Screening Potential DNA Barcode Regions of Chloroplast Coding Genome for Citrus and Its Related Genera

【Objective】 Four coding regions of chloroplast genome of Citrus and its close relatives were analyzed in an attempt to find suitable DNA barcoding markers for species identification and lay a foundation for further study of non-coding region.【Method】 Four chloroplast DNA regions (matK, rpoB, rpoC1 and rbcL ) of 59 Citrus accessions were sequenced, the intergeneric, interspecific, intraspecific genetic distances were calculated, and the phylogenetic tree of all the accessions tested was built based on the distance data obtained.【Result】 The intergeneric and interspecific sequence variations of matK were the highest among four coding regions tested, and had significant difference from other regions studied. On the contrary, no obvious variations were found in the rpoB and rpoC1 regions. The sequence variation of rbcL was medium among the fragments sequenced. 【Conclusion】 The matK sequence could be used as potential candidate fragment for future DNA barcoding study of Citrus and its closely related genera.