龙眼体细胞胚发生中期的蛋白质组学研究

 【目的】了解龙眼体细胞胚发生中期（球形胚至子叶形胚）蛋白质组的变化，为进一步阐明植物体细胞胚发生的机制提供依据。【方法】进行龙眼体细胞胚发生中期的同步化调控，采用双向电泳和质谱对龙眼体细胞胚发生中期的蛋白质组变化进行分析。【结果】龙眼体细胞胚发生中期表达蛋白的种类随着体细胞胚的发育逐渐减少，但在鱼雷形胚阶段其种类数量有所回升，且增加的蛋白分子质量小于66.0 kD，pI主要集中在5.00—7.00。成功鉴定了35个差异蛋白，发现了多个与体细胞胚发生密切相关的蛋白。1/2以上的蛋白与代谢途径有关，多个蛋白与氧化胁迫有关。RAN2 和 GTPase ObgE在龙眼体细胞胚发生中期发生了活跃的变化。【结论】随着体细胞胚的发育，细胞不断分化，体细胞胚中表达的蛋白种类减少。氧化胁迫相关蛋白、RAN2 和 GTPase ObgE与龙眼体细胞胚发生的调控有关。

Proteomics on Somatic Embryogenesis During the Middle Stage in Longan

【Objective】 The changes of proteome patterns during the middle stage of longan (Dimocarpus longan Lour.) somatic embryogenesis were analyzed to provide valuable evidence for further elucidating the mechanism of somatic embryogenesis.【Method】Synchronization of longan somatic embryogenesis during the middle stage was carried out, and changes of proteomic patterns during this period were analyzed by 2-DE and MS.【Result】During this period, diversity of expressed proteins declined with the development of somatic embryos of longan, but it showed a increase at torpedo stage. Newly expressed proteins at torpedo stage included those with a MW less than 66.0 kD and a pI of these proteins range between 5.00 and 7.00. Thirty-five differentially expressed proteins were identified, and many of them were related to somatic embryogenesis.【Conclusion】With the development of somatic embryo, somatic cells differentiated progressively and the amount of expressed proteins decreased. Proteins associated with oxidative stress, RAN2 and GTPase ObgE might be related to regulation of somatic embryogenesis in longan.