奶牛乳房炎金黄色葡萄球菌凝固酶基因型研究

 【目的】了解上海和贵州地区引起奶牛乳房炎的金黄色葡萄球菌凝固酶基因型情况,为奶牛乳房炎的防治提供理论依据。【方法】利用16S rRNA保守序列设计引物,PCR扩增鉴定金黄色葡萄球菌,并利用凝固酶基因及其酶切产物多态性对分离鉴定出的金黄色葡萄球菌进行了凝固酶基因分型。【结果】共鉴定出78株金黄色葡萄球菌,有74株金黄色葡萄球菌扩增凝固酶基因片段,分为5个基因型和两个亚型。PCR 1型为贵州地区优势基因型,PCR 3型为上海地区优势基因型。【结论】两地区各基因型菌株分布比例有显著的地域性差异,这与两地区地理环境和养殖水平差异对病原流行传播的影响有关。

Research on Coagulase Genotyping of Staphylococcus Aureus Isolated from Bovine Mastitis

【Objective】 To study the character on coagulase genotype distribution of Staphylococcus aureus isolated from bovine mastitis in Shanghai and Guizhou areas in China,provide credible theory evidence for prevention and treatment on bovine mastitis. 【Method】 S.aureus strains were identified by PCR amplification on which primer was designed according to the 16S rRNA conserved sequence. The S.aureus strains have been identified were typed by polymorphism of coagulase gene and it’s fragment disposed by incision enzyme. 【Result】 Seventy-eight S.aureus strains were identified and coagulase gene were amplifid out from 74 S.aureus strains. The strains were separated into five genotypes and two subtypes. PCR1 was preponderant genotype of Guizhou area and PCR3 was preponderant genotype of Shanghai area.【Conclusion】The effects on epidemic of the pathogenic bacteria influenced by diversity of geographical conditions and the levels of breeding may be the reasons of distinct diversity in distribution of different genotypic strains in these two areas.