| 检测鸡肉中甲羟孕酮酶联免疫试剂盒的研制 |
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| 引用本文： | 陈飞,冯才伟,余厚美,贾芳芳,杨烁.检测鸡肉中甲羟孕酮酶联免疫试剂盒的研制[J].中国畜牧兽医,2013,40(12):65-69. |
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| 作者姓名： | 陈飞 冯才伟 余厚美 贾芳芳 杨烁 |
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| 作者单位： | 1. 山东春雪食品有限公司, 山东莱阳 265200;2. 北京勤邦生物技术有限公司, 北京 102206 |
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| 摘 要： | 试验旨在利用酶联免疫技术研制一种快速检测鸡肉中甲羟孕酮的试剂盒。经测试，该试剂盒对鸡肉样本的检测限为1 μg/kg，批内、批间相对标准偏差均小于10%；稳定性测试结果表明，试剂盒能在4℃保存12个月；交叉反应率结果表明，单克隆抗体特异性良好。该试剂盒的操作时间仅需45 min，适合现场大量样本的快速检测。
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| 关 键 词： | 甲羟孕酮 酶联免疫试剂盒 鸡肉 快速检测 |
| 收稿时间： | 2013-06-20 |
Development of an Enzyme Linked Immunosorbent Assay Kit for Rapid Detection of Medroxyprogesterone in Chicken |
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| CHEN Fei,FENG Cai-wei,YU Hou-mei,JIA Fang-fang,YANG Shuo.Development of an Enzyme Linked Immunosorbent Assay Kit for Rapid Detection of Medroxyprogesterone in Chicken[J].China Animal Husbandry & Veterinary Medicine,2013,40(12):65-69. |
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| Authors: | CHEN Fei FENG Cai-wei YU Hou-mei JIA Fang-fang YANG Shuo |
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| Institution: | 1. Shandong Spring Snow Food Co., Ltd., Laiyang 265200, China;2. Beijing Kwinbon Biotechnology Co., Ltd., Beijing 102206, China |
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| Abstract: | The experiment was used enzyme immunoassay technique to develop a medroxyprogesterone kit which could rapidly tested in chicken. After testing, the limit of detection was 1 μg/kg in chicken samples,and the intra- and inter-relative standard deviations were less than 10%. The stability test results revealed that the kit could be stored for 12 months at 4℃. The cross-reactive assay showed that the specificity of monoclonal antibody was good. The kit operation time only needed 45 min,which could be suitable for detection of a large number of samples tested rapidly on the spot. |
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| Keywords: | medroxyprogesterone enzyme linked immunosorbent assay kit chicken rapid detection |
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