Expression of short-chain acyl-CoA dehydrogenase during heart development in rats and relationship with cardiac hypertrophy |
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Authors: | ZHOU Si-gui WANG Ping LU Yao YUAN Xi ZANG Lin-quan YANG Zhi-cheng XU Li-peng |
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Institution: | 1.Department of Clinical Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China;2.Institute of New Drug Research, College of Pharmacy, Jinan University, Guangzhou 510632, China;3.Shenzhen Institute for Drug Control, Shenzhen 518029, China;4.Grade 2007,Department of Eight-Year Clinical Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China. |
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Abstract: | AIM: To investigate the expression of short-chain acyl-CoA dehydrogenase during the heart deve-lopment in rats and to analyze the relationship between short-chain acyl-CoA dehydrogenase and cardiac hypertrophy in spontaneously hypertensive rats (SHR). METHODS:The expression and activity of short-chain acyl-CoA dehydrogenase in the hearts of Wistar rats with different ages were measured. Free fatty acids in serum and cardiac muscles were also determined. RESULTS:Compared with the fetal rats of 19 d, the expression and activity of short-chain acyl-CoA dehydrogenase in the postnatal rats of 1 d, 2 weeks, 6 weeks and 16 weeks were increased, and free fatty acids in the serum and myocardium were obviously decreased. The difference began in evidence from the age of 2 weeks. The expression of short-chain acyl-CoA dehydrogenase was significantly up-regulated with negative correlation to free fatty acids in the serum and myocardium during heart development. Systolic blood pressure was similar in 2-week-old SHR and WKY rats, which significantly increased in SHR of 6 weeks and 16 weeks old compared with the age-matched WKY rats. The ratio of left ventricular weight to body weight was markedly elevated in SHR of 2 weeks, 6 weeks and 16 weeks old compared with the age-matched WKY rats, indicating that the appearance of cardiac hypertrophy occurred before the development of hypertension in SHR. Compared with the age-matched WKY rats, the expression and activity of short-chain acyl-CoA dehydrogenase were decreased and free fatty acids in the serum and myocardium were obviously higher in SHR. The expression of short-chain acyl-CoA dehydrogenase was significantly down-regulated with a negative correlation to free fatty acids in the serum and myocardium of SHR. CONCLUSION:The expression of short-chain acyl-CoA dehydrogenase is increased during the heart development, which may be associated with the increase in cardiac fatty acid utilization. The down-regulated expression of short-chain acyl-CoA dehydrogenase in the hypertrophic heart may be responsible for the recapitulation of fetal energy metabolism. |
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Keywords: | Short-chain acyl-CoA dehydrogenase Heart development Fatty acid oxidation Cardiac hypertrophy |
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