| 两种纯化兔抗酸性红73免疫球蛋白G方法的比较研究 |
| |
| 引用本文： | 常向彩,杨晓农,朱子凤,陈瑞,刘凌颖. 两种纯化兔抗酸性红73免疫球蛋白G方法的比较研究[J]. 中国畜牧兽医, 2013, 0(7): 124-126 |
| |
| 作者姓名： | 常向彩 杨晓农 朱子凤 陈瑞 刘凌颖 |
| |
| 作者单位： | 1. 西南民族大学生命科学与技术学院, 四川成都 610041;2. 西南民族大学校医院, 四川成都 610041 |
| |
| 基金项目： | 中央高校基本科研业务费科研平台建设项目(11NPT03);西南民族大学研究生学位点建设项目(2011XWD-S0906)。 |
| |
| 摘 要： | 分别采用饱和硫酸铵法和辛酸—硫酸铵法纯化兔抗酸性红73(acid red 73,AR73)免疫球蛋白G(IgG)。通过二喹啉甲酸(BCA)法、十二烷基硫酸钠—聚丙烯酰胺凝胶电泳(SDS-PAGE)试验、间接ELISA法分别测定IgG的浓度、纯度及效价。结果显示,饱和硫酸铵法所提IgG浓度为36.44 mg/mL,最高效价达1.6×105;辛酸—硫酸铵法所提IgG浓度为15.79 mg/mL,最高效价达3.2×105。提示,辛酸—硫酸铵法IgG纯度优于饱和硫酸铵法。
|
| 关 键 词： | 酸性红73 酸性大红GR 饱和硫酸铵法 辛酸—硫酸铵法 |
| 收稿时间： | 2012-12-07 |
Comparison of Two Methods for Purifying the Rabbit Antibody of Acid Red 73 IgG |
| |
| CHANG Xiang-cai,YANG Xiao-nong,ZHU Zi-feng,CHEN Rui,LIU Ling-ying. Comparison of Two Methods for Purifying the Rabbit Antibody of Acid Red 73 IgG[J]. China Animal Husbandry & Veterinary Medicine, 2013, 0(7): 124-126 |
| |
| Authors: | CHANG Xiang-cai YANG Xiao-nong ZHU Zi-feng CHEN Rui LIU Ling-ying |
| |
| Affiliation: | 1. College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China;2. The Hospital of Southwest University for Nationalities, Chengdu 610041, China |
| |
| Abstract: | To compare the effects of saturated ammonium sulfate method and caprylic acid-ammonium sulfate method for purifying the rabbit anti-AR73 IgG. The IgG were tested in protein content,relative molecular weight, the antibody titer by BCA method,sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and indirect enzyme linked immunosorbent assay (ELISA).The results indicated that the protein content of IgG by saturated ammonium sulfate method was 36.44 mg/mL and the antibody titer was 1.6×105, the protein content of IgG by caprylic acid-ammonium sulfate method was 15.79 mg/mL, and the antibody titer was 3.2×105. The purity of purified antibody by caprylic acid-ammonium sulfate method was higher than that by saturated ammonium sulfate method. |
| |
| Keywords: | acid red 73 acid red GR saturated ammonium sulfate caprylic acid-ammonium sulfate |
|
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
|
点击此处可从《中国畜牧兽医》下载全文 |
|
