大河乌猪磷酸酯转移蛋白基因内含子4的SNPs检测及其与生产性能的关联分析

本研究旨在阐明猪磷酸酯转移蛋白(phospholipid transfer protein,PLTP)基因第4内含子多态性及其与生长性状之间的关系。结果表明,该位点具有2种等位基因A/a,F4、F5、F6各群体中的基因频率分别为0.4172/0.5828、0.4155/0.5845、0.4309/0.5691。取不同基因型纯合子样品进行测序,共发现4个单核苷酸多态位点(SNPs),其有3个突变为转换,1个为颠换,多态位点分别为1085、1192、1286和1305 bp。应用最小二乘模型分析不同基因型对生长性状(初生重、45日龄体重、4月龄体重及6月龄体重、体高、体长、管围、背膘厚)的影响。结果显示,F4代各性状基因型间差异不显著(P>0.05);F5代Aa基因型个体初生重及背膘厚显著高于AA基因型(P<0.05),与aa基因型个体间差异不显著(P>0.05);F6代aa基因型个体4月龄体重及Aa基因型个体6月龄管围都极显著高于AA基因型(P<0.01),aa基因型个体6月龄体高显著高于AA基因型(P<0.05),Aa基因型个体初生重显著高于AA基因型(P<0.05)。另外,Aa基因型的雌性个体对初生重及膘厚性状的影响较AA基因型高(P<0.05);Aa基因型雄性个体对管围性状的影响较AA基因型高(P<0.01)。研究结果提示猪PLTP基因Aa基因型对生长性状有着重要的影响,该SNP可能是改良猪经济性状的重要分子标记位点。

SNPs Detection of PLTP-4 among Dahe Blank Porcine and Association Analysis with their Production Performance

The associations between SNPs in the PLTP gene and production performance of pigs were reported in this study.The results showed alleles detected in the sequence were allele A and a with frequencies of 0.4172 and 0.5828,0.4155 and 0.5845,0.4309 and 0.5691 in F4, F5, F6 generation,respectively. There were 4 mutations of 3 for conversion and 1 for transversion,4 SNPs (1085,1192,1286 and 1305 bp, respectively) were found by the sampling sequence. Associations between the founded Hae Ⅲ RFLP in intron 4 and birth weight, body weight at 45 days, body weight at 4 months, body weight at 6 months, body height at 6 months, body length at 6 months,cannon circumference at 6 months and back-fat thickness at 6 months were tested by least square analysis. In F4 generation,no significant difference between HaeⅢ RFLP and all growth traits(P>0.05). In F5 generation, the birth weight and back-fat thickness at 6 months with Aa genotype were higher than that with AA genotype (P<0.05). In F6 generation,the body weight at 4 months(P<0.01)and body height at 6 months (P<0.05) with aa genotype were higher than that with AA genotype. The cannon circumference at 6 months (P<0.01) and the birth weight (P<0.05) with Aa genotype were higher than that with AA genotype. In addition, the cannon circumference were the highest significant than that with AA genotype male (P<0.01). The birth weight and back-fat thickness at 6 months were higher than that with AA genotype female (P<0.05). These results suggested that the Aa genotype of PLTP gene had some effects on the growth traits of porcine. These SNP might be considered as a potential genetic marker for selection.