Diagnostic value of serum miR-21 for diabetic nephropathy |
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Authors: | ZHU Bing-ming CHEN Wen-jing SU Yun-qin WEN Wang-rong |
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Institution: | Center of Clinical Laboratory Medicine, the First Affiliated Hospital of Jinan University, Guangzhou 510632, China. |
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Abstract: | AIM:To evaluate the diagnostic value of serum miR-21 as a molecular biomarker in the patients with diabetic nephropathy (DN). METHODS:Twenty-five patients with DN, 50 patients with type II diabetes [divided into DMA group and DMB group base on urinary microalbumin (UmAlb), 25 in each group], 25 patients with diabetic nephropathy-induced uremia (UM) and 25 normal controls (NC) were included in the study. Total RNA was extracted from the serum samples for determining the miR-21 level using real-time PCR. The relationship between miR-21 level and clinical parameters of the patients with diabetic nephropathy was analyzed. RESULTS:The relative expression level of serum miR-21 was significantly lower in DN group than that in DMA group, DMB group and NC group, and was significantly higher than that in UM group (all P<001). The serum miR-21 level showed significant negative correlation with cystatin C, UmAlb, UmAlb/urinary creatinine in DN patients (P<0.01,P<0.05,P<0.05). For discriminating the DN patients from NC, DMA, DMB, DMA+NC, DMB+NC and DMA+DMB, the areas under the receiver operating characteristic curve (AUC-ROC) for serum miR-21 were 0848 (95% CI: 0737~0959), 0896 (95% CI: 0812~0980), 0782 (95% CI: 0641~0922), 0838 (95% CI: 0743~0933), 0796 (95% CI: 0675~0917) and 0808 (95% CI: 0704~0911), and the sensitivity and specificity were 800% and 720%, 720% and 880%, 720% and 840%, 760% and 770%, 760% and 820%, 700% and 860%, respectively. For discriminating the DN patients from DMA+DMB+NC, the AUC-ROC was 0845 (95% CI: 0752~0939), and the sensitivity and specificity were 760% and 773%, respectively. CONCLUSION: Serum miR-21 can serve as a molecular diagnostic marker for diagnosis of diabetic nephropathy. |
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Keywords: | miR-21 Diabetic nephropathies Diagnosis |
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