Emodin inhibits proliferation and blocks cell cycle of human breast cancer MCF-7 cells |
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Authors: | LI Meng-jia MA Lian-shun YANG Ya-ping ZHU Wei-wei CHEN Li-xin WANG Li-wei ZHU Lin-yan YANG Hai-feng |
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Institution: | 1.Department of Pharmacology, School of Medicine, 2Analysis and Test Center, 3Department of Physiology, School of Medicine, Jinan University, Guangzhou 510632, China;4.Department of Pathology, Guangdong Provincial Hospital of TCM, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510120, China. |
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Abstract: | AIM:To investigate the effects of emodin on the proliferation of human breast cancer MCF-7 cells and its mechanisms. METHODS:MTT assay was used to observe the viability of MCF-7 cells. The cell cycle distribution and apoptosis of MCF-7 cells was analyzed by flow cytometry. The membrane surface morphology and three-dimensional ultrastructure of MCF-7 cells were observed under atomic force microscope (AFM). RESULTS:MTT assay showed that emodin could inhibit MCF-7 cell proliferation in a dose-dependent manner. Flow cytometric analysis demonstrated that emodin induced cell cycle arrest at G0/G1 phase. Annexin V/PI double staining confirmed that emodin had no effect on cell apoptosis. AFM images revealed that the cell nuclear area was full and the surface of cell membrane was flat and smooth in control group. Compared with control group, the cell nuclear area collapsed and shrank in emodin group at 48 h. The cell membrane ultrastructure showed that the particles in emo-din group had an intensive distribution. The height of cell nuclear area was decreased, and the surface average roughness (Ra) and root mean square roughness (Rq) were elevated in emo-din group compared with control group. CONCLUSION: Emodin has cytotoxicity on MCF-7 cells via cell cycle arrest at G0/G1 phase and ultrastructural changes. |
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Keywords: | Emodin MCF-7 cells Cell proliferation Cell cycle Atomic force microscope |
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