| 以减毒鼠伤寒沙门氏菌为载体的重组NDV-HN口服疫苗的构建与鉴定 |
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| 引用本文： | 田芳华,程相朝,张春杰,李银聚,李静,刘一尘.以减毒鼠伤寒沙门氏菌为载体的重组NDV-HN口服疫苗的构建与鉴定[J].中国畜牧兽医,2013,40(11):59-63. |
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| 作者姓名： | 田芳华 程相朝 张春杰 李银聚 李静 刘一尘 |
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| 作者单位： | 河南科技大学动物科技学院, 河南洛阳 471003 |
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| 基金项目： | 河南科技大学研究生创新基金(CXJJ-YJS-Z005);河南省科技公关项目(112102110019)。 |
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| 摘 要： | 试验旨在构建表达鸡新城疫病毒(NDV)HN基因的重组减毒鼠伤寒沙门氏菌SL1344ΔcrpΔasd(pYA3493-HN)。以pMD18-T-HN为模板,通过PCR扩增出NDV HN基因片段,定向插入原核表达载体pYA3493中,将重组表达质粒pYA3493-HN转入χ6097,再转入减毒鼠伤寒沙门氏菌SL1344ΔcrpΔasd,通过双酶切和PCR对质粒进行鉴定。结果表明,携带NDV HN基因片段的重组减毒鼠伤寒沙门氏菌SL1344ΔcrpΔasd(pYA3493-HN)构建成功。本研究结果为开发鸡新城疫的口服基因工程活载体疫苗奠定了基础。
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| 关 键 词： | 新城疫 HN基因 原核表达载体pYA3493 减毒鼠伤寒沙门氏菌 |
| 收稿时间： | 2012-07-04 |
Construction and Identification of Recombinant Attenuated Salmonella typhimurium Oral NDV-HN Vaccine |
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| TIAN Fang-hua,CHENG Xiang-chao,ZHANG Chun-jie,LI Yin-ju,LI Jing,LIU Yi-chen.Construction and Identification of Recombinant Attenuated Salmonella typhimurium Oral NDV-HN Vaccine[J].China Animal Husbandry & Veterinary Medicine,2013,40(11):59-63. |
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| Authors: | TIAN Fang-hua CHENG Xiang-chao ZHANG Chun-jie LI Yin-ju LI Jing LIU Yi-chen |
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| Institution: | College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China |
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| Abstract: | The study was aimed to construct prokaryotic expression vector of NDV HN gene using attenuated Salmonella typhimurium SL1344ΔcrpΔasd as a carrier. The HN gene was cloned from the plasmid pMD18-T-HN by polymerase chain reaction (PCR) and inserted into the vector pYA3493. Electricity transformation method was used in this recombinant plasmid pYA3493-HN transformating to recepted state Salmonella typhimurium SL1344ΔcrpΔasd. The recombinant plasmid pYA3493-HN was identified by digestion of endonuclease, PCR and sequencing. The results confirmed that recombinant attenuated Salmonella typhimurium vaccine SL1344ΔcrpΔasd(pYA3493-HN) was constructed successfully, which provided a foundation for research and development of NDV HN orally genetic engineering live vaccine. |
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| Keywords: | Newcastle disease HN gene prokaryotic expression vector pYA3493 attenuated Salmonella typhimurium |
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