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Effect of vitamin C on expansion of CD4+ effector memory T cells in vitro
Authors:ZHANG Yi-wen  LUO Hai-hua  ZHANG Xu  ZHANG Hui  LIU Chao
Institution:Institute of Human Virology, Key Laboratory of Tropical Disease Control of Ministry of Education, Sun Yat-sen University, Guangzhou 510080, China.
Abstract:AIM:To investigate the effect of vitamin C (VC), a small-molecule compound, on the expansion of CD4+ effector memory T cells (TEM) in vitro and to improve the effect of adoptive immunotherapy. METHODS:Normal human peripheral blood CD4+ T-lymphocytes were isolated and randomly divided into 2 groups: control group and experiment group. VC was added into experimental group. Thereafter, the expansion of CD4+ TEM in the 2 groups was detected by cell counter and flow cytometry. RESULTS:VC did not significantly affect the total number of CD4+ T cells, while it raised the ratio of TEM in CD4+ T cells at an optimal concentration of 100 mg/L. After 10 days of the expansion of CD4+ T cells, the number of CD4+ TEM in experiment group was (3.56±0.35)×106, significantly larger than that in control group [(1.22±0.15)×106, P<0.01]. CONCLUSION: VC can effectively promote the expansion of CD4+ TEM in vitro, which provides a simple, safe and effective expansion method for adoptive immunotherapy.
Keywords:Vitamin C  Effector memory T cells  Adoptive immunotherapy  
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