沙蒿多糖组合制剂对滩羊羔羊瘤胃菌群多样性的影响

本试验旨在研究沙蒿多糖不同组合制剂对滩羊羔羊瘤胃菌群多样性的影响.选择健康、体重相近的断奶滩羊公羔60只,按体重随机分为4个组,每组15只羊.对照组饲喂基础饲粮,试验1组、试验2组和试验3组分别饲喂在基础饲粮中添加5.5 g/d沙蒿多糖+5.5 g/d包被丁酸钠、5.5 g/d沙蒿多糖+5.5 g/d丁酸甘油酯和5.5...

Effects of Artemisia Polysaccharide Combination Preparation on Rumen Microflora Diversity of Tan Lambs

This experiment was conducted to evaluate the effects of Artemisia polysaccharide combination preparation on rumen microflora diversity of Tan lambs.Sixty healthy male Tan lambs with similar body weight were randomly divided into four groups according to body weight,each group contained 15 lambs.Lambs in the control group were fed a basal diet,and others in trial group 1,trial group 2 and trial group 3 were fed basal diets supplemented 5.5 g/d Artemisia polysaccharide+5.5 g/d coated sodium butyrate,5.5 g/d Artemisia polysaccharide+5.5 g/d butyrate glyceride and 5.5 g/d Artemisia polysaccharide+1.1 g/d monensin,respectively.The pre-experimental period lasted for 15 days,and the experimental period lasted for 60 days.The results showed as follows:1)compared with the control group,the concentrations of acetic acid,butyric acid and total volatile fatty acids and proportion of acetic acid in rumen fluid of Tan sheep of trial group 1 and trial group 2 were significantly increased(P<0.05).2)The Chao1 index of trial group 2 was significantly higher than that of the control group(P<0.05),and the Shannon index and Simpson index of trial group 2 were trend higher than the control group(0.05≤P<0.10).3)At phylum level,compared with the control group,the relative abundance of Tenericutes in rumen fluid of trial group 1 was significant increased(P<0.05),and the relative abundances of Firmicutes and Fibrobacteres were trend to increase(0.05≤P<0.10);the relative abundance of Chloroflexi in rumen fluid of trial group 2 was significant increased(P<0.05),and the relative abundance of Verrucomicrobia was trend to increase(0.05≤P<0.10);the relative abundances of Cyanobacteria and Synergistetes in rumen fluid were significant increased(P<0.05).4)At genus level,compared with the control group,the relative abundance of Rikenellaceae_RC9_gut_group in rumen fluid of trial group 1 was significant decreased(P<0.05),and the relative abundances of Succiniclasticum and Solobacterium were significant increased(P<0.05);the relative abundance of Sharpea in rumen fluid of trial group 2 was significant decreased(P<0.05),and the relative abundance of Pseudobutyrivibrio was significant increased(P<0.05);the relative abundances of Rikenellaceae_RC9_gut_group and Sharpea in rumen fluid of trial group 3 were significant decreased(P<0.05),and the relative abundance of Syntrophococcus was trend to increase(0.05≤P<0.10).In conclusion,dietary Artemisia polysaccharide combination preparation can increase the microflora diversity and improve the rumen microflora of Tan lambs,and the effect of adding Artemisia polysaccharide+coated sodium butyrate is the best.