大豆卵磷脂对马精液冷冻保存的效果研究

为探索适宜浓度的大豆卵磷脂(soybean lecithin,SL)代替卵黄(egg yolk,EY)对马精液冷冻保存的效果,本试验分别以5%卵黄(V/V)和10%、20%、30%大豆卵磷脂(m/V)作为精液的冷冻保护剂冷冻解冻马精液,解冻后分别对精子细胞的运动参数、精子细胞膜的完整性、精子细胞脂质氧化物丙二醛的值和线粒体膜完整性进行检测。结果发现,精液冷冻解冻后,5%卵黄和10%、20%、30%大豆卵磷脂对精子的总运动精子数无显著影响(P>0.05),但30%大豆卵磷脂具有最大的原地摆动精子数和最小的直线前进精子数(P<0.05),其他运动参数差异不显著(P>0.05);20%大豆卵磷脂代替卵黄后具有最高的质膜完整性,同时产生最少的脂质氧化物丙二醛;线粒体膜电位经流式细胞仪检测时,30%大豆卵磷脂活精子高膜电位数最高,但与20%大豆卵磷脂之间差异不显著(P>0.05)。试验结果表明,20%大豆卵磷脂能够代替卵黄作为冷冻保护剂用于马精液的冷冻保存。

Study on the Effect of Soybean Lecithin in the Cryopreservation of Stallion Semen

The objective of this study was designed to determine the effect of soybean lecithin replace of egg yolk in the cryopreservation of stallion semen. After collecting, stallion semen was divided into four aliquots. We took 5% egg yolk (EY,V/V) as control group, and the treatment groups were different concentration soybean lecithin (SL,m/V):10%, 20%,30%. Frozen-thawed semen was evaluated the spermatozoa motility parameters, sperm plasma and mitochondrial membrane integrity, lipid oxidative production MDA. The results showed that the total motility was not significant compared 5% egg yolk to different concentration soybean lecithin (P>0.05). 30% soybean lecithin obtained the highest local motility and lowest progress motility (P<0.05). While 20% soybean lecithin produced the lowest MDA in all the groups, and had a better sperm plasma integrity. It also found that 20% and 30% soybean lecithin were higher in JC-1⁺/PI^-, but compared with 20 soybean lecithin had no effect (P>0.05). It was concluded that 20% soybean lecithin could instead of 5% egg yolk in the cryopreservation of stallion semen.