Screening and Positioning of Three Chromosome-specific BAC Clones in Gossypium raimondii |
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| Authors: | QIN Qin LIU Fang GAN Yi-Mei WANG Chun-Ying WANG Yu-Hong CAI Xiao-Yan WANG Kun-Bo |
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| Affiliation: | 1. Institute of Cotton Research Chinese Academy of Agricultural Sciences／State Key Laboratory of Cotton Biology, Anyang, 455000, China; 2. The Institute of Tropical Bioscience and Biotechnology of Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture(China), Haikou, Hainan, 571101, China |
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| Abstract: | We screened a bacterial artificial chromosome(BAC) library of Gossypium barbadense acc. Pima 90-53 to identify chromosome-specific BAC clones. Using BAC-fluorescence in situ hybridization technology, we obtained three BAC clones specific to chromosome D501, D502, and D510 of Gossypium raimondii, which could be used as cytological markers for those three chromosomes. Comparative mapping of these three BACs between G. barbadense and in G. raimondii showed that these three BAC clones could also be used to identify chromosomes Db01, Db02, and Db10 in G. barbadense. The position of the BAC clone 280G06 on chromosome 10 did not show colinearity between G. barbadense and G. raimondii, possibly because of chromosomal rearrangement. |
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| Keywords: | G. raimondii individual chromosome bacterial artificial chromosome(BAC) Fluorescence in situ hybridization (FISH) |
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