| 小鼠髓源未成熟树突状细胞的分离与培养 |
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| 引用本文： | 廖娟红,林祥梅,贾广乐. 小鼠髓源未成熟树突状细胞的分离与培养[J]. 中国畜牧兽医, 2013, 0(7): 107-111 |
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| 作者姓名： | 廖娟红 林祥梅 贾广乐 |
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| 作者单位： | 中国检验检疫科学研究院, 北京 100121 |
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| 基金项目： | 中国检验检疫科学研究院基本科研业务费专项(2010JK007)。 |
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| 摘 要： | 本试验旨在建立一种体外诱导培养小鼠未成熟树突状细胞(dendritic cell,DC)的方法。应用重组粒细胞-巨噬细胞集落刺激因子(rGM-CSF)在体外诱导小鼠骨髓前体细胞分化为未成熟树突状细胞,进行形态学观察、细胞表型分析、刺激T细胞增殖等方法,对小鼠髓源未成熟树突状细胞的体外诱导培养进行鉴定。试验结果显示,小鼠骨髓来源的DC在体外培养8 d后,特异性细胞表面标志CD11c的表达量达到81.09%,中度表达MHCⅡ,低表达CD40、CD80、CD86。本试验成功地建立了体外小鼠髓源DC扩增的方法。
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| 关 键 词： | 小鼠 树突状细胞 骨髓 |
| 收稿时间： | 2012-12-10 |
Isolation and Culture of Mouse Bone Marrow-derived Dendritic Cells |
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| LIAO Juan-hong,LIN Xiang-mei,JIA Guang-le. Isolation and Culture of Mouse Bone Marrow-derived Dendritic Cells[J]. China Animal Husbandry & Veterinary Medicine, 2013, 0(7): 107-111 |
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| Authors: | LIAO Juan-hong LIN Xiang-mei JIA Guang-le |
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| Affiliation: | Chinese Academy of Inspection and Quarantine, Beijing 100121, China |
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| Abstract: | To establish a method of isolation, cultivation and purification of dendritic cells from mouse bone marrow in vitro. Mononuclear cells isolated form mouse bone marrow were induced into immature dendritic cells by being cultured with rGM-CSF and then examined from aspects of morphology, phenotype and function of T lymphocyte proliferation. The result showed that a large number of typical dendritic cells were observed after culturing for 9 days, and high expressed CD11c 81.09%, middle exproessed MHCⅡ, low expressed CD40, CD80, CD86. A method to generate large number of DCs from mouse bone marrow in vitro had been established. |
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| Keywords: | mouse dendritic cells bone marrow |
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