Anti-inflammatory effect of huperzine A on protection of rat neural stem cells in vitro |
| |
Authors: | ZHU Ning LIN Ji-zong CHEN Qing-zhuang WEI Mei-dan WANG Yong |
| |
Affiliation: | 1.Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China;2.Department of Hepatobiliary Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China;3.Department of Pharmacy, the Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, China. |
| |
Abstract: | AIM:To explore the anti-inflammatory effect of huperzine A (HupA) and its neuroprotective effect on rat neural stem cells (NSCs). METHODS:The microglia and NSCs were isolated from neonatal rat hippocampal tissues and co-cultured in a Transwell system. The cells were divided into 3 groups:control group, amyloid beta-peptide (Aβ) group and HupA group. The microglia layer in Aβ group was treated with Aβ1-42 (10 μmol/L), while that in HupA group was pretreated with HupA (1 μmol/L) before Aβ1-42 stimulation. The culture supernatant levels of inflammatory mediators, including interleukin 6 (IL-6), tumor necrosis factor α (TNF-α) and macrophage inflammatory protein 1α (MIP-1α), were detected by LiquiChip technique. The apoptosis of NSCs was determined by flow cytometry and Western blotting. RESULTS:The microglia secreted a large number of inflammatory mediators with the stimulation of Aβ. In Aβ group, the levels of IL-6, TNF-α and MIP-1α were significantly higher than those in control group at 72 h (P<0.01), and the apoptotic rate of NSCs was 25.46% (P<0.01). In HupA group, the concentrations of IL-6, TNF-α and MIP-1α decreased significantly as compared with Aβ group (P<0.01), and the apoptotic rate of NSCs was only 8.05% (P<0.01). The Bcl-2/Bax ratio in HupA group was higher than that in Aβ group (P<0.05). CONCLUSION:Huperzine A reduces the secretion of cytokines and chemokines, and attenuates microglia-mediated neuroinflammation, thus protecting NSCs against inflammation-induced apoptosis. |
| |
Keywords: | Huperzine A Microglia Neural stem cells Inflammation Neuroprotective effect Amyloid beta-peptides |
|
| 点击此处可从《园艺学报》浏览原始摘要信息 |
|
点击此处可从《园艺学报》下载全文 |
|