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| Cry1Ac 青花菜对小菜蛾的抗性和产卵行为的影响 | |
| 引用本文： | 崔树松,仪登霞,杨丽梅,方智远,刘玉梅,庄木,张扬勇,孙培田. Cry1Ac 青花菜对小菜蛾的抗性和产卵行为的影响[J]. 中国蔬菜, 2013, 1(16): 43 |
| 作者姓名： | 崔树松 仪登霞 杨丽梅 方智远 刘玉梅 庄木 张扬勇 孙培田 |
| 作者单位： | （中国农业科学院蔬菜花卉研究所，北京100081） |
| 基金项目： | 国家自然科学基金项目（31071697），国家大宗蔬菜产业技术体系项目（nycytx-35-gw01），“十一五”863 计划项目 （2008AA10Z155），农业部园艺作物遗传改良重点开放实验室项目（ICS，CAAS）（1610032011011） |
| 摘 要： | 利用3 个Cry1Ac 蛋白表达量不同的青花菜株系，研究了其对抗性小菜蛾和敏感小菜蛾的抗 性和产卵选择行为的影响。实验室结果表明：在非Bt 青花菜对照上，抗性小菜蛾和敏感小菜蛾的存活率 差异不显著；在Cry1Ac 蛋白表达量为167 ng·g-1 的Bt 青花菜上，敏感小菜蛾的死亡率达到100%，而抗 性小菜蛾的死亡率仅为18%；在Cry1Ac 蛋白表达量为224 ng·g-1 和246 ng·g-1 的Bt 青花菜上，抗性小 菜蛾和敏感小菜蛾的死亡率均达到100%。抗性小菜蛾和敏感小菜蛾在不同Cry1Ac 蛋白表达量的Bt 青花 菜上的产卵量没有显著差异，表明Cry1Ac 蛋白表达量对小菜蛾的产卵行为没有影响。温室试验结果与实 验室结果基本一致。 |
| 关 键 词： | Bt 基因 青花菜 小菜蛾 产卵行为 抗性治理 |
Effects of Cry1Ac Broccoli on Resistance to Diamondback Moth andOviposition Behavior |
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| CUI Shu-Song,YI Deng-Xia,YANG Li-Mei,FANG Zhi-Yuan,LIU Yu-Mei,ZHUANG Mu,ZHANG Yang-Yong,SUN Pei-Tian. Effects of Cry1Ac Broccoli on Resistance to Diamondback Moth and Oviposition Behavior[J]. China Vegetables, 2013, 1(16): 43 |
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| Authors: | CUI Shu-Song YI Deng-Xia YANG Li-Mei FANG Zhi-Yuan LIU Yu-Mei ZHUANG Mu ZHANG Yang-Yong SUN Pei-Tian |
| Affiliation: | （Institute of Vegetables and Flowers， Chinese Academy of Agricultural Sciences， Beijing 100081， China） |
| Abstract: | In this study，effects of Bacillus thuringiensis（Bt）broccoli expressing different levels of Cry1Ac protein on resistance to Cry1Ac-susceptible and Cry1A c-resistant diamondback moth（DBM） and oviposition behavior were evaluated. The concentrations of Cry1Ac protein in these 3 Bt broccoli lines were 167 ng·g-1，224 ng·g-1，and 246 ng·g-1 fresh weight，respectively．Untransformed broccoli plants were used as control．The larval survival rate of diamondback moth on control leaves was not significantly different between resistant and susceptible DBM. The mortality of Cry1Ac-resistant DBM was 18% on Bt plants expressing 167 ng·g-1 Cry1Ac protein．The Cry1Ac-susceptible larvae could not survive on all of the Bt broccoli．There was no significant difference in number of eggs laid by DBM adults among different Bt broccoli．The development of Cry1Ac-susceptible and Cry1Ac-resistant DBM on Bt plants was also tested in greenhouse. The results of greenhouse trial were similar to that of laboratory tests. |
| Keywords: | Bacillus thuringiensis Broccoli Plutella xylostella Oviposition behavior Resistance management |
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