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Role of hepatitis B virus in intrahepatic TGF-β1/Smads signaling pathway
Authors:ZHANG Xiang-mei  LE Xiao-hua  CHEN Pei-fen  GOU Ji-zhou  SUN Yan  ZHONG Xun-zhen  ZHOU Ya-min  LIU Xiao-ling  CHEN Qing-shan
Institution:1.Department of Pathology, the Third Peoples Hospital of Shenzhen, Shenzhen 518112, China;2.Epidemiology Division, School of Medicine, Jinan University, Guangzhou 510632, China.
Abstract:AIM:To explore the effects of hepatitis B virus (HBV) on intrahepatic expression of transforming growth factor β1(TGF-β1) and Smads. METHODS:The expression of intrahepatic TGF-β1, HBsAg and HBcAg in control group and chronic hepatitis B (CHB) group was detected by immunohistochemical method.The serum HBV DNA content was determined by real-time PCR. The role of HBV in the expression of TGF-β1, Smad3 and Smad7 in human hepatic stellate cell line LX-2 in vitro was observed by cell culture and Western blotting. RESULTS:The average score of intrahepatic TGF-β1 expression in CHB group was higher than that in control group. With the increase in serum HBV DNA content, intrahepatic TGF-β1 expression was also enhanced. In the HBcAg positive hepatic tissue, there was higher TGF-β1 expression than that in the liver tissue of HBcAg negative. Compared with control group and HBV+anti-TGF-β1 group, HBV caused increased expression of TGF-β1 and Smad3 in HBV group in vitro. No difference of Smad7 protein among control group, HBV group and HBV+anti-TGF-β1 group was observed. CONCLUSION: The expression of intrahepatic TGF-β1 is related to serum HBV DNA and hepatocellular HBcAg in the patients with CHB. HBV-induced liver fibrosis mainly relies on positive regulatory mechanisms of Smad3,and the negative regulation by Smad7 almost does not function.
Keywords:Hepatitis B virus  Transforming growth factor β1  Smad proteins  Liver fibrosis  
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