Effects of diosmin on activity of myeloperoxidase in renal tissues and expression of CD11b,CD54 and CD62L on neutrophils in rats with kidney ischemia and reperfusion

AIM：To observe the effects of diosmin on the activity of myeloperoxidase (MPO) in renal tissues and the expression of CD11b, CD54 and CD62L on neutrophils in rats with kidney ischemia and reperfusion. METHODS：One hundred and eighty Sprague-Dawley rats were randomly divided into 3 groups: sham operation (SO) group, ischemia and reperfusion (I/R) group and diosmin+I/R (DOSM+I/R) group. At the end of the experiment, renal tissues were obtained and the activity of MPO was detected by ELISA. The expression of CD11b, CD54 and CD62L on polymorphonuclear leukocytes was determined by flow cytometry after monoclonal antibody labeling. RESULTS：The renal MPO activity at different time points in DOSM+I/R group was significantly lower than that in I/R group (P<0.01 or P<0.05). The expression levels of CD11b, CD54 and CD62L on polymorphonuclear leukocytes in I/R group were significantly higher than those in SO group (P<0.01 or P<0.05), while those in DOSM+I/R group were lower than those in I/R group (P<001 or P<0.05). CONCLUSION: Diosmin not only decreases the activity of MPO in renal tissues, but also reduces the expression of CD11b, CD54 and CD62L on polymorphonuclear leukocytes, suggesting that its protective effect against kidney ischemia-reperfusion injury through anti-inflammatory mechanism.