氟对小鼠附睾中成熟精子超微结构的影响

本试验探讨了氟对小鼠附睾中成熟精子超微结构的影响,为氟的生殖毒性研究与检测提供依据。选取8周龄性成熟雄性昆明小鼠20只,随机分为4组,对照组小鼠饮用蒸馏水,低、中和高氟组小鼠分别饮用含25、50和100 mg/L氟化钠的蒸馏水,于45 d后断颈处死小鼠,取小鼠附睾尾,经2.5%戊二醛固定后,采用透射电镜观察小鼠附睾中成熟精子的超微结构变化。与对照组相比,低氟组小鼠精子头部质膜断裂,部分脱落,个别线粒体肿胀、形态模糊;中氟组精子头部质膜脱落,顶体部分缺失,线粒体形状不规则,嵴间腔扩大;高氟组精子头部质膜脱落,线粒体排列不规则,出现空泡化,嵴结构模糊。结果表明,氟暴露小鼠附睾中成熟精子头、尾部中段均有不同程度的结构改变,尤以线粒体出现较明显的异常,且氟浓度越高,精子超微结构损伤越严重。

Effect of Fluoride on the Ultrastructure of Epididymal Mature Sperm in Male Mice

The experiment was conducted to study the effects of fluorine on ultrastructure of mature sperm in mice epididymis, and provide the basis for reproductive toxicity study and detection induced by fluorine. 20 healthy male Kunming mice were randomly distributed into 4 groups including the control group(distilled water), low dose fluoride (25 mg/L NaF) in their drinking water,middle dose fluoride (50 mg/L NaF)and high dose fluoride (100 mg/L NaF)group. After 45 days exposure, all mice were sacrificed by cervical dislocation and cauda epididymidis were collected for further analysis. 2.5% glutaraldehyde had been used to fix cauda epididymidis, the ultrastructure change of normal and different doses of fluorosis mice sperm were observed by the transmission electron microscope. Compared with the control group, plasmalemma around the head of sperm fractured, plasmalemma partly had fallen off, some of the mitochondria swelled and the structure of mitochondria was vague in low dose fluoride group. The plasmalemma of mitochondria had fallen off, acrosome partly lost, the figure of mitochondria was irregular and mitochondrial intracristal space was enlarged in middle dose fluoride group. For high dose fluoride group, the plasmalemma of mitochondria had fallen off, irregularly arranged mitochondrion acrosome dilated, mitochondrial vacuolization and mitochondrial cristae was vague. The results showed that varying degrees of changes had occurred in the ultrastructure of both the sperm head and the middle of sperm tail, especially mitochondria displayed obvious structural abnormalities, and the higher the fluorine concentration, the more dramatic changes had taken place on the ultrastructure of epididymal mature sperm in male mice.