甘草提取物对绵羊皮下脂肪沉积与代谢相关酶活性的影响

作者旨在探讨添加不同水平甘草提取物(LE)对绵羊皮下脂肪沉积及代谢相关酶活性的影响。采用单因素试验设计,选用健康、断奶后20~30 d、初始体重相近的宁夏滩羊公羔50只,随机分为5组,每组10只,其中对照组饲喂基础日粮,不添加LE;试验Ⅰ~Ⅳ组分别在基础日粮中添加1000、2000、3000、4000 mg/kg LE。试验期120 d,试验结束后进行屠宰并测定滩羊皮下脂肪重、血清脂质代谢相关指标及皮下脂肪代谢相关酶活性。结果表明,与对照组相比,试验Ⅰ~Ⅳ组的皮下脂肪重分别降低了20.16%、14.40%、18.11%、20.16%;添加3000 mg/kg LE降低血清总胆固醇的效果最明显,LE对总甘油三酯(TG)和高密度脂蛋白(HDL-C)含量均无显著影响(P>0.05);LE显著降低皮下脂肪组织葡萄糖-6-磷酸脱氢酶(G6PDH)活性(P<0.05),其中添加3000 mg/kg LE降低G6PDH活性的效果最佳,对脂肪酸合成酶(FAS)、苹果酸脱氢酶(MDH)、异柠檬酸脱氢酶(ICDH)和6-磷酸脱氢酶(6PGDH)活性的影响均未达到显著水平(P>0.05)。提示,LE有降低血清总胆固醇和皮下脂肪重的趋势,其中添加3000 mg/kg LE的降脂作用最好,甘草提取物主要通过下调脂肪合成途径的关键酶活性来抑制绵羊皮下脂肪沉积,但甘草提取物调控滩羊皮下脂肪代谢关键酶的机制,尚需进一步研究。

Effects of Liquorice Extract on Subcutaneous Fat Deposition and Activities of Metabolism Related Enzymes in Sheep

This study was conducted to investigate the effects of liquorice extract on subcutaneous fat deposition and the activities of metabolism related enzymes in sheep. In this single-factor experiment,50 healthy male lamb of Ningxia Tan sheep on 20 to 30 d after weaning with similar initial body weight were randomly divided into 5 groups and experiment Ⅰ to Ⅳ groups were fed with diets supplemented with 0,1000,2000,3000,4000 mg/kg basic ration liquorice extract (LE),respectively. After a trial period of 120 days,the sheep were slaughtered and the subcutaneous fat weight,serum lipid metabolism indices and subcutaneous fat metabolism related enzyme activity were tested. Results showed that compared with control group,the subcutaneous fat weight of trialⅠ to Ⅳ groups reduced by 20.16%,14.40%,18.11% and 20.16%,respectively. The effect of 3000 mg/kg LE was the best to decrease the serum total cholesterol(TC). But LE had no significant effect on total triglycerides (TG) and high density lipoprotein cholesterol (HDL-C) levels (P>0.05). The glucose 6-phosphate dehydrogenase (G6PDH) activity in subcutaneous adipose tissue was significantly suppressed by LE (P<0.05),and 3000 mg/kg LE was the best. But the influence of LE on the activities of fatty acid synthase (FAS),malic dehydrogenase(MDH),isocitrate dehydrogenase(ICDH) and 6-phosphate dehydrogenase (6PGDH) had no significant effect (P>0.05).Thus,LE tended to reduce the subcutaneous fat weight and serum total cholesterol, and 3000 mg/kg LE adding level had the best lipid-lowering effect,and LE reduced fat deposition mainly by down regulating the key enzymes in fat synthesis approach,however,the specific regulation mechanism of LE on subcutaneous fat metabolism key enzymes in sheep still needed to further study.