布氏艾美耳球虫保定株的鉴定及致病性研究

本研究采用琼脂薄膜球虫单卵囊分离技术,从河北省保定市暴发球虫病的某鸡场鸡粪便中成功分离出布氏艾美耳球虫卵囊。生物学特性研究结果表明,该虫种潜隐期为125 h,卵囊大小为26.1 μm×21.3 μm,形状指数为1.22,寄生与病变部位主要在小肠后段、直肠和泄殖腔,特征性病变是肠黏膜层有出血斑纹及血性内容物。感染一定剂量该球虫,鸡的生产性能受到影响,当感染剂量为2×10⁵个/羽时会出现鸡死亡,表明该虫株具有较强致病性;应用特异PCR方法对获得的卵囊进行分子鉴定,结果确认所分离和纯化的球虫卵囊为布氏艾美耳球虫纯种,并将该虫株命名为布氏艾美耳球虫保定株--BBD株。

Study on Indentification and Pathogenicity of Baoding Strain of Eimeria brunetti

In this study, the oocysts of E.brunetti were isolated from chicken faeces in Baoding city, Hebei province by single-oocyst isolation technique. The results of biological characteristics indicated that the minimum prepatent period was 125 h for the E.brunetti strain, with an average size of 26.1 μm×21.3 μm, and a shape index of 1.22. This parasite could cause hemorrhage of intestinal mucosa in rectum, cloaca, and the posterior part of small intestine. Infected with certain dose of this parasite, the performance trait of chickens were affected severe, and 2×10⁵ per chicken oocysts could cause chickens to die, which indicated that the parasite had a relatively strong pathogenicity. The oocysts were further identified using specific PCR assays, and the results illustrated that the samples collected from single oocyst separation were mono-specific species of E.brunetti, which was named as BBD strain.