白花蛇舌草多糖对小鼠免疫功能和生长发育的影响

为探讨白花蛇舌草多糖对小鼠免疫功能和生长发育的影响,本研究将60只雌性昆明小鼠随机等分为4组,1~3组分别以50、100和150 mg/kg 体重的白花蛇舌草多糖粗提物灌胃小鼠,连续给药7 d,第4组以等量的生理盐水灌胃,作为空白对照组。最后一次给药12 h后,各组小鼠用鸡新城疫弱毒疫苗接种,2头份/只,分别于给药结束后的7、14和21 d采小鼠外周血并分离淋巴细胞和血清。用淋巴细胞转化试验(CCK-8法)测定外周血淋巴细胞增殖能力;微量血凝抑制试验评价血清中新城疫抗体水平;测定各组小鼠体重;21 d测定小鼠腹腔巨噬细胞吞噬能力,并测定脾脏指数。统计学分析结果显示,白花蛇舌草多糖组小鼠的外周血淋巴细胞增殖能力、免疫抗体水平、腹腔巨噬细胞吞噬能力、脾脏指数和体重均显著高于对照组(P<0.05),且具有量效关系。结果表明,白花蛇舌草多糖能显著提高机体的特异性免疫和非特异性免疫功能,促进机体的生长发育。本试验结果为利用白花蛇舌草多糖开发保健产品提供了参考。

Effect of Hedyotis diffusa Willd Polysaccharide on Immune Function and Growth in Mice

To investigate the effect of Hedyotis diffusa Willd polysaccharide (HDP) on immune function and growth of mice,a total of 60 mice were randomly divided into 4 groups. Group 1 to 3 were orally administered with HDP at a dose of 50, 100 and 150 mg/(kg·BW) for 7 days, respectively. The mice of control group were orally administered with the same volume of saline. Mice of each group were inoculated with Newcastle disease vaccine at 12 h after the last oral administration. Peripheral blood was sampled to separate leukomonocyte and sera on the 7th,14th and 21th day after the last oral administration. Lymphocytic proliferation was evaluated using CCK-8 method, and serum antibody titers of Newcastle disease were measured by micro-hemagglutination inhibition test. Meanwhile, mice of each group were weighed. Macrophage phagocytic function test and spleen coefficient method were assayed on the 21th day after the last oral administration. The statistic results showed that HDP could significantly enhance the level of lymphocytes proliferative capacity, serum antibody, peritoneal macrophage phagocytosis, the index of spleen and weight in mice (P<0.05). These results demonstrated that HDP could significantly increase the non-specific and specific immune function, and improve the weight of body in mice. The results in this study provided new insight into the development of novel type of health product using HDP.