| ENO1过表达对籽鹅卵泡颗粒细胞凋亡基因Bcl-2表达的影响 |
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| 引用本文： | 王江璐,计红,张伟宏,赫荣贺,郭景茹,郭丽,杨焕民. ENO1过表达对籽鹅卵泡颗粒细胞凋亡基因Bcl-2表达的影响[J]. 中国畜牧兽医, 2013, 40(7): 10-13 |
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| 作者姓名： | 王江璐 计红 张伟宏 赫荣贺 郭景茹 郭丽 杨焕民 |
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| 作者单位： | 黑龙江八一农垦大学动物科技学院, 黑龙江大庆 163319 |
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| 基金项目： | 黑龙江省自然科学基金重点项目(ZD20116)。 |
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| 摘 要： | 本试验将原代培养的籽鹅卵泡颗粒细胞转染ENO1腺病毒过表达载体,应用实时荧光定量PCR方法检测ENO1过表达对颗粒细胞Bcl-2 mRNA表达量的影响。结果发现ENO1过表达使卵泡颗粒细胞Bcl-2 mRNA表达量极显著增加(P<0.01)。提示ENO1可能通过使Bcl-2基因表达量增加,从而抑制籽鹅卵泡颗粒细胞凋亡,促进卵泡的发育。
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| 关 键 词： | 籽鹅 ENO1过表达 Bcl-2基因 颗粒细胞 |
| 收稿时间： | 2012-11-09 |
Effects of ENO1 Overexpression with Adenovirus Vector on Bcl-2 in the Granulose Cell of Zi Goose |
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| WANG Jiang-lu,JI Hong,ZHANG Wei-hong,HE Rong-he,GUO Jing-ru,GUO Li,YANG Huan-min. Effects of ENO1 Overexpression with Adenovirus Vector on Bcl-2 in the Granulose Cell of Zi Goose[J]. China Animal Husbandry & Veterinary Medicine, 2013, 40(7): 10-13 |
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| Authors: | WANG Jiang-lu JI Hong ZHANG Wei-hong HE Rong-he GUO Jing-ru GUO Li YANG Huan-min |
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| Affiliation: | College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China |
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| Abstract: | The paper was to study the effects of ENO1 overexpression with adenovirus vector on Bcl-2 in the granulose cell of Zi goose. Adenovirus vector with ENO1 were transfected to Zi goose primary cultured granulose cells, and Bcl-2 mRNA of granulose cells were detected by the method of Real-time fluorescence quantitative PCR. The results showed that ENO1 overexpression made the Bcl-2 mRNA expression in the granulose cells significantly increase (P<0.01). ENO1 overexpression made the primary culture follicle granulose cells in vitro improve Bcl-2 mRNA expression, which suggested that ENO1 might inhibit the apoptpsis of Zi goose granulose cell. |
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| Keywords: | Zi goose ENO1 overexpression Bcl-2 gene granulosa cells |
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