Role of caveolae in high glucose-induced extracellular matrix production in rat mesangial cells |
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Authors: | LU Sheng-xia LIU Yuan-tao KONG feng DU Yue-juan LIU Ye FU Yu-qin |
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Institution: | 1.Department of Nephrology,2Department of Endocrinology,3Central Laboratory, the Second Hospital of Shandong University, Jinan 250033, China;4.Department of Nephrology, Central Hospital of Jinan City, Jinan 250013, China. |
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Abstract: | AIM:To investigate the role of caveolae in high glucose (HG)-induced extracellular matrix (ECM) production in rat mesangial cells (MCs). METHODS:Synchronized rat MCs were divided into normal glucose group, HG group, HG+methyl-β-cyclodextrin (β-MCD) group and HG+β-MCD+cholesterol (Chol) group. Western blotting was used to detect the protein expression of caveolin-1 (Cav-1), phosphorylated caveolin-1 (p-Cav-1-Y14) and collagen type 1 (Col I). The mRNA expression of Cav-1 was determined by real-time PCR. ELISA was used to measure the level of fibronectin (FN) in the supernatant. RESULTS:High glucose significantly increased the expression of FN and Col I. In HG 12, 24 and 48 h groups, the mRNA and protein levels of Cav-1 were not significantly different from those in HG 0 h group, whereas the level of p-Cav-1-Y14 was significantly increased. β-MCD significantly attenuated HG-induced elevation of p-Cav-1-Y14 and FN production, but had no effect on HG-induced Col I expression. All these responses to β-MCD were abolished by Chol. CONCLUSION:High glucose significantly increases the production of Col I and FN in rat MCs. FN production induced by high glucose is mediated by p-Cav-1-Y14. |
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Keywords: | Caveolin-1 Fibronectin Collagen type I |
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