猪输卵管特异性糖蛋白结构与功能的生物信息学分析

利用Lasergene、Protscale、SOPMA、Mega、ScanProsite等生物信息学软件对猪输卵管特异性糖蛋白(oviduct-specific glycoprotein,OGP)进行物理性质、结构及系统进化分析。结果表明,猪OGP cDNA包含1个1581 bp的开放阅读框,编码527个氨基酸,有信号肽,属于分泌蛋白,无明显疏水区;猪OGP蛋白二级结构主要是α-螺旋和不规则卷曲。系统进化分析结果表明,猪OGP蛋白与绵羊OGP亲缘关系最近,与牛OGP的亲缘关系较远。提示,OGP蛋白属于糖苷水解酶18(glycosyl hydrdase 18 family,GH18)家族,牛OGP的GH18结构与猪的差别较大。

Bioinformatics Analysis of Porcine Oviduct-specific Glycoprotein Structure and Function

Lasergene,Protscale,SOPMA,Mega and ScanProsite were used to study the structure, conservation and phylogeny of porcine oviduct-specific glycoprotein (OGP). The results showed that the full length of porcine OGP contained a complete open read coding of 1581 bp, which encoded 527 amino acids. The porcine OGP had obvious signal peptide and it was a secretion protein, it was no obvious hydrophobic region. The secondary structures were mainly composed of α-helix and random coli. Phylogenetic tree analysis of the amino acids sequences showed that the porcine OGP displayed nearest relationship with the sheep OGP, and lower homology with those of bovine. The OGP was a member of glycosyl hydrolase 18 family (GH18). The GH18 domain structure of OGP had high differences between bovine and porcine.