MEF2A/2D regulates depolarization induced differentiation via Rho signaling pathway in VSMCs |
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Authors: | REN Jing-li WANG Yan-mei SONG Guo-hua KANG Hong-yu SUN Ming-zhen |
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Institution: | Key Laboratory of Biomedical Engineering, Luohe Medical College, Luohe 462000, China. |
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Abstract: | AIM:To investigate the role of myocyte enhance factor 2 (MEF2) in the regulation of depolarization-induced differentiation via Rho signaling pathway in vascular smooth muscle cells (VSMCs). METHODS: In primarily cultured mouse portal vein VSMCs, the techniques of immunofluorescence, Western blotting, real-time RT-PCR and siRNA transfection were applied to determine the membrane translocation of RhoA, the phosphorylation of LIM kinase (LIMK) and cofilin-2, and the mRNA expression of 4 MEF2 isoforms, myocardin and SM22α under high KCl-induced depolarization. RESULTS:RhoA membrane translocation occurred 30 s after depolarization, while phosphorylation of LIMK and cofilin-2 peaked at 10 min and 30 min, with increment of 42.20% and 32.75%, respectively. The mRNA expression of MEF2A and 2D was increased by 47.63% and 48.15%, respectively. In MEF2A/2D knockdown VSMCs, the mRNA expression of myocardin was not sensitive to depolarization, while SM22α expression was not affected. CONCLUSION:MEF2A/2D, acting on myocardin, is involved in the regulation of depolarization-induced differentiation via Rho signaling pathway in VSMCs |
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Keywords: | Myocyte enhancer factor 2 Depolarization Myocardin Vascular smooth muscle cells |
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