Effect of Xueshuantong injection on LPS-induced DIC in rabbits

AIM：To evaluate the effect of Xueshuantong injection on lipopolysaccharide (LPS)-induced experimental disseminated intravascular coagulation (DIC) in rabbits. METHODS：To establish the LPS-induced DIC model, LPS was continuously injected into the rabbit autricular vein for 6 h. The survival rate of the rabbits was recorded after 24 h. The plasma levels of alanine aminotransferase (ALT) and blood urine nitrogen (BUN) were detected. Activated partial thromboplastin time (APTT), prothrombin time (PT), platelet count and fibrinogen concentration were measured. The activity of protein C, antithrombin Ⅲ (ATⅢ) and the concentration of tumor necrosis factor α (TNF-α) were also determined. RESULTS：The survival rate of DIC rabbits was only 26.7%. BUN and ALT increased obviously. APTT and PT became much longer, platelet count, fibrinogen, protein C and ATⅢ decreased obviously, and plasma TNF-α increased remarkably. The intravenous administration of Xueshuantong injection increased the survival rate of DIC rabbits up to 66.7% in a dose-dependent manner. Xueshuantong injection also decreased the plasma levels of BUN, ALT, APTT, PT and TNF-α significantly, while increased the levels of fibrinogen, platelet, protein C and ATⅢ in plasma. CONCLUSION： Xueshuantong injection has therapeutic effect on LPS-induced DIC in rabbits.