Role of autophagy in intervertebral disc degeneration in diabetic rats |
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Authors: | ZHENG Xu-hao ZHANG Xiao-lei JIANG Li-bo HU Xu-qi JIN Yong-long ZHENG Yi-jing WU Wei XU Hua-zi |
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Institution: | Department of Spine Surgery, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China. |
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Abstract: | AIM:To explore the levels of apoptosis and autophagy in the nucleus pulposus tissues of intervertebral discs in diabetic rats. METHODS:Sixteen weeks after injection of streptozocin (STZ), the lumbar intervertebral discs were obtained from the rats. The histological changes were observed by hematoxylin-eosin (HE) staining and alcian blue staining. The apoptosis of the nucleus pulposus cells was measured by the methods of terminal deoxynucleotidyl transferase-mediated nick-end labeling (TUNEL), immunohistochemistry, and Western blotting. The level of autophagy in the nucleus pulposus cells was detected by Western blotting and immunohistochemistry. RESULTS:Compared with normal group, HE and alcian blue staining suggested that the intervertebral discs of the diabetic rats became degenerate. The expression of caspase-3 and the apoptotic rate were increased in intervertebral disc nucleus pulposus of the diabetic rats. The results of immunohistochemistry and Western blotting showed that the expression levels of LC3Ⅱ/LC3Ⅰand beclin-1 in the diabetic rats were higher than those in normal group. CONCLUSION: The STZ-induced diabetes accelerates degeneration of the intervertebral discs. In addition, the apoptosis and autophagy are increased in the intervertebral discs of diabetic rats. |
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Keywords: | Intervertebral disc degeneration Diabetes mellitus Autophagy Apoptosis |
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