猪繁殖与呼吸综合征病毒感染猪肺脏组织环腺苷酸磷酸二酯酶的活性及其mRNA表达变化

试验旨在探究在猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)感染过程中环腺苷酸(cAMP)特异性磷酸二酯酶(PDE)的活性及其mRNA表达量的变化,以期为PDE抑制剂的应用提供相关依据。试验采集PRRSV感染猪肺脏组织,运用高效液相色谱(HPLC)法检测cAMP在PDE反应前后的变化,计算cAMP-PDE活性;通过Real-time PCR检测cAMP-PDE mRNA表达量的变化。结果显示,PRRSV感染猪肺脏组织中cAMP-PDE活性显著高于对照组(P<0.05),在检测的8个PDE亚型中,除PDE4A外,PDE4B、PDE4C、PDE4D、PDE7A、PDE7B、PDE8A、PDE8B mRNA的表达量均高于对照组。结果表明,cAMP-PDE在PRRSV感染引起的猪肺脏部的炎症反应过程中存在异常变化,提示cAMP-PDE特异性抑制剂有望减轻PRRSV感染引起的猪肺脏部炎症损伤。

Activity and mRNA Expression of Cyclic Nucleotide-specific Phosphodiesterase in the Lung of Porcine Infected with Porcine Reproductive and Respiratory Syndrome Virus

The objective of this paper was to study the activity and expression change of cAMP-PDE in lung of porcine infected with porcine reproductive and respiratory syndrome virus (PRRSV) through the examination of PDE activity and gene expression,and provided data for the application of PDE inhibitors basis.Acquisition lung of porcine infected with PRRSV,cAMP-PDE activity was calculated by cyclic nucleotides content change examined with HPLC before and after the PDE reaction,and cAMP-PDE mRNA expression was detected by Real-time PCR. The results showed that the activity of cAMP-PDE extracted from lung of PRRS group was significantly higher than that of the control group (P<0.05), and in addition to PDE4A, mRNA expression of PDE4B, PDE4C, PDE4D, PDE7A, PDE7B, PDE8A and PDE8B were higher than that of the control group.Those results indicated that there were some abnormal changes of cAMP-PDE in the inflammation progress of lungs infected by PRRSV,and suggested that specific inhibitor of the cAMP-PDE might ease the inflammation injury in lung of porcine infected with PRRSV.