Effects of FHL1 on proliferation and migration of rat pulmonary arterial smooth muscle cells stimulated by cigarette smoke |
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Authors: | PU Gui-mei YANG Li LI Yu-ping CHEN Cheng-shui JIANG Lei |
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Affiliation: | 1.Department of Respiratory Medicine, 2Longwan Medical Laboratory, the First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China. |
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Abstract: | AIM:To explore the role of four-and-a-half LIM domain 1 (FHL1) in the proliferation and migration of rat distal pulmonary arterial smooth muscle cells (PASMCs) stimulated by cigarette smoke extract (CSE). METHODS:Rat distal PASMCs were isolated and primarily cultured. The cells were divided into 6 groups: blank control group, negative transfection group, FHL1 siRNA transfection group, CSE group, CSE + negative transfection group and CSE + FHL1 siRNA transfection group. The mRNA and protein expression of FHL1 was detected by real-time PCR and Western blotting, respectively. Cell proliferation and migration were determined by CCK-8 and Transwell assays, respectively. RESULTS:CSE promoted the proliferation and migration of PASMCs, and increased the expression of FHL1 protein (P<0.01), but did not change the expression of FHL1 mRNA (P>0.05). FHL1 siRNA transfection attenuated the proliferation and migration of PASMCs induced by CSE (P<0.01). CONCLUSION: FHL1 protein is involved in CSE-induced proliferation and migration of rat PASMCs. |
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Keywords: | FHL1 protein Smoking Pulmonary arterial smooth muscle cells |
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