Effect of microRNA-100 on proliferation activity and cell cycle of hepatocarcinoma cells |
| |
Authors: | JI Xiao-hui FAN Bing-lin ZHANG Hong-ge CAI Xin-hua ZHU Wu-ling |
| |
Institution: | School of Basic Medical Science, Xinxiang Medical University, Xinxiang 453003, China. |
| |
Abstract: | AIM:To investigate the effect of microRNA-100 (miR-100) on the proliferation activity and cell cycle of hepatocarcinoma cells. METHODS:Synthetic miR-100 mimic and its negative control were transfected into human hepatocarcinoma HepG2 cells by liposome method. After transfection, the cell counting kit-8 (CCK-8) was used to measure the cell proliferation activity. The cell cycle distribution was determined by flow cytometry. The expression of Polo-like kinase 1 (Plk1) at mRNA and protein levels was detected by quantitative real-time PCR (qRT-PCR) and Western blotting. RESULTS:The transfection efficiency mediated by cationic liposome was greater than 85%. The inhibitory rates of cell proliferation in HepG2 cells were (43.5±12.2)%, (46.5±3.7)% and (52.1±0.2)% at 24 h, 48 h and 72 h after transfected with miR-100 mimic, respectively, which were significantly increased as compared with the control cells. Moreover, the cell proliferation index in experimental group (35.8 ± 1.4) was higher than that in negative control group (39.2 ± 1.0) and simple liposome group (40.7 ± 2.0) at 72 h. At the same time, the mRNA and protein expression levels of Plk1 obviously decreased in HepG2 cells transfected with miR-100 at 72 h after transfection. CONCLUSION:miR-100 suppresses the proliferation activity of hepatocarcinoma cells by down-regulating Plk1 gene expression. |
| |
Keywords: | Liver neoplasms MicroRNA-100 Polo-like kinase 1 |
|
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
|