不同剂量链脲佐菌素及不同性别大鼠糖性白内障模型的比较研究

本试验旨在研究不同剂量链脲佐菌素(streptozocin,STZ)及不同性别在建立大鼠糖性白内障模型方面的差异。将65、70和75 mg/kg 3个浓度STZ通过一次性腹腔注射建立雌、雄性大鼠糖性白内障模型,36 h后尾静脉采血测血糖,3周后进行裂隙灯检查,每天测量动物饮食量及排粪尿量变化,每周测定体重。试验结果显示,70 mg/kg STZ比65 mg/kg STZ糖性白内障发生时间缩短约1周,且比75 mg/kg STZ死亡率低。同浓度STZ腹腔注射后雌性大鼠糖尿病发生时间为72 h,雄性大鼠为36 h,白内障发生时间雌性比雄性大鼠慢约1周。试验结果表明,腹腔注射70 mg/kg STZ在糖性白内障模型建立所需时间及模型稳定性等方面具有优势,雄性大鼠较雌性更易于建立糖性白内障模型。

Comparative Study of Different Doses of Streptozocin and Different Genders in Establishing Diabetic Cataract Models of Rats

This assay was aimed to study the differences of different doses of streptozocin(STZ) such as 65,70 and 75 mg/kg,and different genders in establishing diabetic cataract models of rats.Single intraperitoneally injection of different doses of STZ to establish male and female diabetic cataract.We measured blood sugar after 36 h of injection, examined diabetic cataract after three weeks, measured food and drink consumption every day and weight changes every week, etc. The results showed that 70 mg/kg STZ was faster than 65 mg/kg STZ in establishing diabetic cataract,and had lower motality compared with 75 mg/kg STZ.We found that 70 mg/kg STZ was more superiority than the other two doses in the time required for the appearance of cataract, model stability and other aspects. Besides,male rats was more suitable in establishing diabetic cataract models.