首页 | 本学科首页   官方微博 | 高级检索  
     检索      


Effects of docosahexaenoic acid on large-conductance calcium-activated potassium channels in rat pulmonary artery smooth muscle cells
Authors:CHEN Rui  LIU Pei-jing  YAN Jin-chuan  GU Yu-chun
Institution:1.Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China;2.Institute of Molecular Medicine, Peking University, Beijing 100871, China.
Abstract:AIM:To investigate the effects of docosahexaenoic acid (DHA) on large-conductance calcium-activated potassium channels (BKCa) in rat pulmonary artery smooth muscle cells (PASMCs).METHODS:BKCa currents in individual PASMCs were recorded by patch-clamp technique in whole-cell configuration.Calcium sparks in PASMCs caused by DHA were recorded by confocal microscopy. RESULTS:DHA activated BKCa . BKCa current densities were (30.5±6.5)pA/pF,(59.4±5.8)pA/pF, (87.2±4.3)pA/pF and (117.3±7.1) pA/pF (P<0.01) with the addition of DHA at concentrations of 0, 0.1, 1 and 10 μmol/L, respectively. Hypoxia inhibited BKCa currents in PASMCs, but this inhibition was reversed by DHA (10 μmol/L). DHA (10 μmol/L) induced an increase in [Ca2+]i with a maximal increase rate of (71.9±4.1)%. CONCLUSION:DHA activates BKCa in rat PASMCs, leading to the vasodilation of pulmonary arteries.
Keywords:Pulmonary artery smooth muscle cells  Docosahexaenoic acid  Large-conductance calcium-activated potassium channels  Hypoxia  
点击此处可从《园艺学报》浏览原始摘要信息
点击此处可从《园艺学报》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号