Feulgen-DNA densitometry of embryo sacs permits discrimination between sexual and apomictic plants in Paspalum simplex |
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Authors: | Maria Eugenia Caceres Fulvio Pupilli Camilo Luis Quarín Sergio Arcioni |
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Institution: | (1) Istituto di Ricerche sul Miglioramento Genetico delle Piante Foraggere del CNR, Via della Madonna alta, 130 I-06128 Perugia, Italy |
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Abstract: | The chromosomes of sexual diploid plants of Paspalum simplex were colchicine-doubled and the plant obtained were crossed with their aposporous natural tetraploid counterparts to generate
a F1 population segregating for apomixis. Analysis of the DNA content during megagametogenesis indicated that although the
nuclei of nucellus and developing embryo sacs were in both the G1 and G2 phases, polar nuclei and egg cells of mature embryo
sacs tended to remain in the G1 phase. Because both meiotic and aposporous mature embryo sacs are of the 8-nucleated-type
in P. simplex and are barely distinguishable, nuclear DNA content of polar nuclei was used to distinguish apomictic and sexual phenotypes
and confirmation obtained by progeny testing.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | apomixis nuclear DNA content Paspalum simplex progeny tests |
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