| 鸡MyoG基因在成纤维细胞中的表达 |
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| 引用本文： | 张婷婷,胡兰,王宏艳,袁亚琦,郝文博,杨晓宇. 鸡MyoG基因在成纤维细胞中的表达[J]. 东北农业大学学报, 2010, 41(3) |
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| 作者姓名： | 张婷婷 胡兰 王宏艳 袁亚琦 郝文博 杨晓宇 |
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| 作者单位： | 沈阳农业大学畜牧兽医学院,沈阳,110161;沈阳农业大学畜牧兽医学院,沈阳,110161;沈阳农业大学畜牧兽医学院,沈阳,110161;沈阳农业大学畜牧兽医学院,沈阳,110161;沈阳农业大学畜牧兽医学院,沈阳,110161;沈阳农业大学畜牧兽医学院,沈阳,110161 |
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| 基金项目： | 辽宁省教育厅科研基金资助项目(2007304) |
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| 摘 要： | 利用分子克隆技术,通过RT-PCR反映扩增、克隆、测序含有Eco RⅠ/XhoⅠ酶切位点的MyoG片段,构建真核荧光表达载体pEGFP-N1-MyoG,并通过脂质体瞬时转染鸡成纤维细胞,对表达产物进行RT-PCR和SDS-PAGE鉴定。结果表明,经脂质体介导重组体pEGFP-N1-MyoG可成功转染鸡成纤维细胞,RT-PCR和SDS-PAGE电泳证实pEGFP-N1-MyoG可在鸡成纤维细胞中表达出MyoG蛋白。该结果为进一步研究MyoG基因的生物学作用奠定了基础。
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| 关 键 词： | MyoG 基因克隆 真核表达 |
Expression in chick embryo fibroblast cell of MyoG gene |
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| ZHANG Tingting,HU Lan,WANG Hongyan,YUAN Yaqi,HAO Wenbo,YANG Xiaoyu. Expression in chick embryo fibroblast cell of MyoG gene[J]. Journal of Northeast Agricultural University, 2010, 41(3) |
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| Authors: | ZHANG Tingting HU Lan WANG Hongyan YUAN Yaqi HAO Wenbo YANG Xiaoyu |
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| Affiliation: | College of Animal Sciences;Shenyang Agricultural University;Shenyang 110161;China |
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| Abstract: | MyoG cDNA containing Eco R Ⅰ/Xho Ⅰ sites was amplified by RT-PCR, and then cloned and sequenced. Eukaryotic expression vector pEGFP-N1-MyoG was transfected into chick embryo fibroblast cells with lipidosome, and the expression protein was detected by SDS-PAGE. The result showed that MyoG gene was cloned into pEGFP-N1 successfully, and its expression at mRNA and protein level was detected with RT-PCR and SDS-PAGE. The experiment established the basis for further study on the function of MyoG gene. |
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| Keywords: | MyoG transfection eukaryotic expression |
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