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无芒隐子草花序不同部位种子萌发期光照需求及抗旱性综合评价
引用本文:陶奇波,董小兵,朱清文,白梦杰,孙秋瑾,韩云华,王彦荣.无芒隐子草花序不同部位种子萌发期光照需求及抗旱性综合评价[J].草地学报,2017(5):993-1001.
作者姓名:陶奇波  董小兵  朱清文  白梦杰  孙秋瑾  韩云华  王彦荣
作者单位:草地农业生态系统国家重点实验室农业部草牧业创新重点实验室草业科学国家级实验教学示范中心兰州大学草地农业科技学院,甘肃兰州,730020
基金项目:国家重点基础研究发展项目(973项目),长江学者与创新团队发展计划,高等学校学科创新引智计划(111计划),兰州大学中央高校基本科研业务费专项资金,草地农业生态系统国家重点实验室基本科研业务费资助
摘    要:以无芒隐子草(Cleistogenes songorica)花序不同部位的种子为材料,采用聚乙二醇(PEG-6000)模拟不同水势(0.0,-0.2,-0.4,-0.6,-0.8 MPa)研究种子萌发期光照需求并进行抗旱性综合评价。结果表明:花序各部位种子的发芽率、发芽势、发芽指数以及活力指数等指标随着渗透势的降低而减小。适度的干旱胁迫促进胚根生长,然而干旱胁迫对胚芽生长则始终起到抑制作用。无芒隐子草花序各部位种子在萌发过程中均需要光照条件,在黑暗环境下其种子发芽率、发芽势等指标均显著下降,胚根长度与胚芽长度亦显著低于光照条件。用模糊数学隶属法综合评价各部位种子的抗旱性,结果表明其抗旱性自高至低依次为:中部下部上部顶部,鞘藏小穗中种子的抗旱性远高于顶穗中的种子,在无芒隐子草的建植中应该优先考虑。

关 键 词:无芒隐子草  花序部位  干旱胁迫  光照  种子萌发

Light Requirement and Evaluation of Drought Resistance of Cleistogenes songorica Seeds from Different Inflorescence Positions at Germination Stage
Abstract:The performance of Cleistogenes songorica seed germination and early seedling growth of the seeds harvested from different inflorescence positions were evaluated under different water potential (0.0,-0.2,-0.4,-0.6,-0.8 MPa) and light regimes.The Fuzzy Mathematics Based Comprehensive Evaluation method was used to investigate the drought tolerance of the seed harvested from different inflorescence positions.The results showed that germination percentage, germination energy, germination index and vigor index reduced significantly with the decline of osmotic pressures.Moderate osmotic stress significantly increased the radicle length, while under the tested osmotic stress treatmetns plumule length was reduced significantly.Seed germination and seedling growth were significantly decreased in darkness compare with that of under light condition.The results of analysis of drought tolerance using Fuzzy Mathematics Based Comprehensive Evaluation suggested that seeds from four positions in seed germination ranked as:Middle> Bottom> Upper> Top spike.Seeds from internodes spike should be highly recommend ensuring the successful establishment of C.songorica.
Keywords:Cleistogenes songorica  Inflorenscence positions  Drought stress  Light  Seed germination
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