| 自制间接法猪瘟抗体ELISA检测试剂盒性能研究 |
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| 引用本文： | 田锦,李志敏,王茂臻.自制间接法猪瘟抗体ELISA检测试剂盒性能研究[J].北京农业职业学院学报,2019,33(5):17-21. |
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| 作者姓名： | 田锦 李志敏 王茂臻 |
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| 作者单位： | 北京农业职业学院,北京,102442;北京农学院,北京,102206;北京市育英学校,北京,100036 |
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| 基金项目： | 北京农业职业学院科技研发推广类项目(XY-YF-16-30)；北京市农委对接服务新型生产经营主体科技能力提升项目(20170203-14) |
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| 摘 要： | 为了评价实验室自行研发的间接法猪瘟抗体ELISA检测试剂盒的性能，我们用调试后的ELISA试剂盒和两个进口试剂盒(法国LSI公司及美国IDEXX公司产品)，分别对184份临床样品进行了检测。结果显示:实验室初步建立的检测体系与LSI公司试剂盒符合率为83.6%、特异性100%、敏感性80.6%，与IDEXX公司试剂盒符合率为76.7%、特异性62%、敏感性93%。
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| 关 键 词： | 猪瘟 ELISA检测 自制试剂盒 |
| 收稿时间： | 2019/2/25 0:00:00 |
Performance of Swine Fever Antibody ELISA Test Kit by Self-developedIndirect Method |
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| TIAN Jin,LI Zhi-min,WANG Mao-zhen.Performance of Swine Fever Antibody ELISA Test Kit by Self-developedIndirect Method[J].Journal of Beijing Agricultural Vocation College,2019,33(5):17-21. |
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| Authors: | TIAN Jin LI Zhi-min WANG Mao-zhen |
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| Institution: | Beijing Vocational College of Agriculture, Beijing 102442, China;Beijing University of Agriculture,Beijing 102206, China; Beijing Yuying School, Beijing 100036, China |
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| Abstract: | In order to evaluate the performance of swine fever antibody ELISA test kit by the indirect
method developed by our laboratory, one ELISA kit and two imported kits (products of France LSI
Company and American IDEXX Company) were used to test 184 clinical samples. The results show that
the compliance rate of our initial established test system is up to 83.6% with the kit from LSI Company,
the specificity is 100%, and the sensitivity is 80.6%; the compliance rate with the kit from IDEXX
Company is 76.7%, the specificity is 62%, and the sensitivity is 93%. |
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| Keywords: | swine fever ELISA test self-made kit |
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