马铃薯CrRLK1Ls基因家族的鉴定及响应晚疫病菌信号的表达分析

Catharanthus roseus receptor-like kinase1-like kinases (CrRLK1Ls)是植物所特有的一类蛋白类受体激酶,在维持细胞壁完整性、细胞间通讯、生物和非生物应激反应中发挥重要作用。本研究鉴定了马铃薯CrRLK1Ls (StCrRLK1Ls)基因家族的成员数量,并对其理化特性、染色体位置、进化特征、亚细胞定位和对晚疫病菌侵染时的表达模式进行全面分析。结果显示,共获得17个StCrRLK1Ls基因,其氨基酸序列大小为753～997 aa,分子量和等电点分别为83.34～108.69kD和5.30～7.56,主要位于质膜。进化分析将马铃薯、拟南芥、水稻、苹果和番茄的CrRLK1Ls家族成员分为7个亚组,马铃薯CrRLK1Ls家族成员分布于亚组Ⅲ、Ⅳ、Ⅴ、Ⅵ和Ⅶ。StCrRLK1Ls不均匀的分布于8条染色体上,存在3个串联重复基因簇,包含6个基因。此外,StCrRLK1Ls启动子区域存在响应植物激素、防卫和逆境等多种顺式调控元件。‘大西洋’和‘陇薯7号’接种晚疫病菌(Phytophthora infestans,Pi)后,大量StCrRLK1...

Genome-wide identification and expression patterns in response to signals from Phytophthora infestans of CrRLK1Ls gene family in potato

Catharanthus roseus receptor-like kinasel-like kinases(CrRLK1Ls),a class of plant-specific protein receptor kinases,play an important role in maintaining cell wall integrity,cell-to-cell communications,and biotic and abiotic stress response.In this study,StCrRLK1Ls family members were identified,and their physical and chemical characteristics,chromosome location,evolutionary characteristics,subcellular location,and the expression patterns were comprehensively analyzed.The results showed that17 StCrRLK1Ls members were detected,with amino acid size(753-997 aa),molecular weight(83.34-108.69 kD),and isoelectric point(5.30-7.56).Among them,StCrRLK1Ls were mainly predicted on plasma membrane.Evolutionary analysis indicated that CrRLK1Ls from potato,rice,apple,Arabidopsis,and tomato could be divided into seven subgroups,and StCrRLK1Ls were distributed in subgroups Ⅲ,Ⅳ,Ⅴ,Ⅵ, and Ⅶ.Moreover,StCrRLK1Ls were distributed on eight chromosomes,and there are three tandem repeat gene clusters,including six members.In addition,there were many cis-elements in the promoter region of StCrRLK1Ls,which mainly responded to hormones,defense,and stress signals.After the inoculation with Phytophthora infestans(Pi),a large number of StCrRLK1Ls were differentially expressed in Atlantic and Longshu 7,and the relative expression levels of StCrRLK1L8 and StCrRLK1L10 were obviously up-regulated during Pi infection.The relative expression levels of StCrRLK1L11 were 9.74 times compared with the control at eight days Atlantic inoculated Pi.We speculated that they could be used as the candidate genes for disease resistance and further functional analysis,which might play an important role in response to pathogenic fungi of potato.