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转sck和hpt基因对水稻不育系及其组合农艺性状的影响
引用本文:何龙飞,莫长明,李创珍,卢升安,王爱勤,易小平.转sck和hpt基因对水稻不育系及其组合农艺性状的影响[J].分子植物育种,2008,6(2):227-232.
作者姓名:何龙飞  莫长明  李创珍  卢升安  王爱勤  易小平
作者单位:广西大学农学院,南宁,530004
基金项目:国家转基因植物研究和产业化专项基金 , 广西青年科学基金 , 广西科技攻关项目 , 广西新世纪十百千人才工程基金
摘    要:本研究以通过基因枪转化法获得的保持系D62BS-1、2为供体,不育系D62A为受体进行hpt、sck的杂交转移,获得转sck的不育系,并以转育成的不育系D62AS进行杂交配组,对它们进行了遗传表达、农艺性状、叶绿素含量、净光合速率、干物质生产特性、抗虫性的检测.结果表明:sck和hpt能够保持紧密连锁,杂交转移到杂种F1中,但也有极少数的hpt,sck没有实现转移;D62AS及其所配杂交组合的农艺性状、叶绿素含量、净光合速率、干物质生产特性基本保持不变;外源sck基因在杂种F2代按孟德尔单基因显性3:1的方式分离.钻蛀性螟虫危害的水稻白穗植株PCR均检测出阳性株.

关 键 词:转sck基因水稻不育系  农艺性状  遗传表达  基因对  水稻不育系  杂交组合  农艺性状  影响  Line  Male  Sterility  Rice  Agronomic  Characteristics  Transgenic  阳性  植株  白穗  螟虫  分离  单基因  孟德尔  杂种  连锁  结果

Effects of Transgenic hpt and sck on Agronomic Characteristics of Rice Male Sterility Line and its Combinations
He Longfei,Mo Changming,Li Chuangzhen,Lu Sheng'an,Wang Aiqin,Yi Xiaoping.Effects of Transgenic hpt and sck on Agronomic Characteristics of Rice Male Sterility Line and its Combinations[J].Molecular Plant Breeding,2008,6(2):227-232.
Authors:He Longfei  Mo Changming  Li Chuangzhen  Lu Sheng'an  Wang Aiqin  Yi Xiaoping
Institution:He Longfei Mo Changming Li Chuangzhen Lu Sheng'an Wang Aiqin Yi Xiaoping College of Agronomy,Guangxi University,Nanning,530004
Abstract:Transgenic male sterility line D62AS was gained with maintainer line D62BS-1 and D62BS-2 as provider, male sterility line D62A as receptor, hybrid combination was made with D62AS as male sterility line. D62AS-1 and its genetic expression, agronomic characteristic, chlorophyll content, net photosynthetic rate, dry matter accumulation, rice lesf roller resistance were analyzed. The results showed that sck gene kept close catenation with hpt gene, they could be transferred to F1, but there were some exceptions...
Keywords:Transgenic sck male sterility line  Agronomic characteristic  Genetic expression  
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