| 棣棠花组织培养与快速繁殖技术研究 |
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| 引用本文： | 张茹琴.棣棠花组织培养与快速繁殖技术研究[J].安徽农业科学,2009,37(17):7877-7878. |
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| 作者姓名： | 张茹琴 |
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| 作者单位： | 青岛农业大学农学与植物保护学院,山东青岛,266109 |
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| 摘 要： | 目的]提高棣棠花育苗繁殖速度。方法]以棣棠花嫩茎为外植体，进行组织培养与快速繁殖研究。结果]在MS＋2．0mg／L 6-BA＋0．2mg／L NAA培养基中，簇生芽分化数最多，质量最好，分化率这100％；在MS＋1．0mg／L 6-BA＋0．5mg／L KT＋0．1mg/L NAA培养基中，增殖倍数最高，为11．87；在1／2MS＋0．1mg／L NAA培养基中生根效果最好，每个嫩茎基部有3～5条根；炼苗成功后移栽成活率达96％。结论]诱导簇生芽的最佳培养基为NS＋2．0mg／L 6-BA＋0．2mg／L NAA，继代培养的最佳培养基为MS＋1．0mg／L 6-BA＋0．5mg/L KT＋0．1mg／L NAA，再生苗在1／2 MS＋0．1mg／LNAA的培养基上生根效果较好。
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| 关 键 词： | 棣棠花 嫩茎 组织培养 快速繁殖 |
Study on the Tissue Culture and Rapid Multiplication of Kerria japonica |
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| ZHANG Ru-qin.Study on the Tissue Culture and Rapid Multiplication of Kerria japonica[J].Journal of Anhui Agricultural Sciences,2009,37(17):7877-7878. |
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| Authors: | ZHANG Ru-qin |
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| Institution: | ZHANG Ru-qin (College of Agronomy , Plant Protection,Qingdao Agricultural University,Qingdao,Sh,ong 266109) |
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| Abstract: | Objective] The research aimed to improve the nursery propagation rate of Kerria japonica.Method] Tender stem of K.japonica was used as explants to investigate the rapid propagation technology.Result] In MS+2.0 mg/L 6-BA+0.2 mg/L NAA medium,the number of clustered bud differentiation was the highest,the differentiation rate reached to 100%,and the quality of clustered bud was the best;In MS+1.0 mg/L 6-BA+0.5 mg/L KT+ 0.1 mg/L NAA medium,the proliferation multiples of the bud was the highest,reached 11.87;... |
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| Keywords: | Kerria japonica Tender stem Tissue culture Rapid propagation |
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