| 基于SSR优化反应体系的茄子亲子鉴定 |
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| 引用本文： | 王利英,乔军,石瑶,李素文.基于SSR优化反应体系的茄子亲子鉴定[J].山西农业科学,2011,39(8):782-785,790. |
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| 作者姓名： | 王利英 乔军 石瑶 李素文 |
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| 作者单位： | 天津科润蔬菜研究所,天津,300384 |
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| 基金项目： | 天津师范大学开放实验室项目 |
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| 摘 要： | 利用分子标记SSR建立快速、准确可靠的纯度检测技术是控制杂交种纯度的有效措施。通过对茄子SSR反应体系进行优化,从40对备选引物中进一步筛选共显性多态标记,开展茄子的分子标记鉴定研究,试验结果显示,有7对SSR多态标记在2个杂交种及亲本中表现一致,且为共显性,条带清晰,可以作为茄子的亲子鉴定标记,便于明确父母本来源及杂交种纯度,提高育种效率。
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| 关 键 词： | 亲子鉴定 SSR 反应体系 茄子 |
Paternity Test of Eggplant (Solanum melongena L.) Based on Optimized SSR Reaction System |
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| WANG Li-ying,QIAO Jun,SHI Yao,LI Su-wen.Paternity Test of Eggplant (Solanum melongena L.) Based on Optimized SSR Reaction System[J].Journal of Shanxi Agricultural Sciences,2011,39(8):782-785,790. |
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| Authors: | WANG Li-ying QIAO Jun SHI Yao LI Su-wen |
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| Institution: | WANG Li-ying,QIAO Jun,SHI Yao,LI Su-wen(Tianjin Kernel Vegetable Research Institute,Tianjin 300384,China) |
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| Abstract: | To establish rapid and accurate purity detection technique using SSR marker is an effective measure for eggplant seed purity control.Seven primers were selected from 40 pairs of SSR,which gave stable and polymorphic amplification profiles.The amplification bands of hybrids were complementary with that of the parents.The SSR identification system established in this paper could be used to identify parents of eggplant and purity of hybrids seed rapidly for breeding efficiency improvement. |
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| Keywords: | paternity test SSR reaction system eggplant |
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