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| 猪伪狂犬病病毒的分离鉴定 | |
| 引用本文： | 杨庆芳,宁官保,李俊达. 猪伪狂犬病病毒的分离鉴定[J]. 山西农业科学, 2011, 39(8): 886-889. DOI: 10.3969/j.issn.1002-2481.2011.08.34 |
| 作者姓名： | 杨庆芳 宁官保 李俊达 |
| 作者单位： | 1. 晋中职业技术学院,山西晋中,030600 2. 山西农业大学,山西太谷,030801 3. 武乡县畜牧兽医中心,山西武乡,046300 |
| 摘 要： | 从山西某猪场发病猪的脑组织中分离到1株疑为猪伪狂犬病病毒(PRV)毒株,通过BHK-21细胞培养、病毒毒力滴定、中和试验、PCR检测病毒方法对该分离株进行了鉴定,结果发现:分离的病毒在BHK-21细胞培养盲传4代后出现典型细胞病变;用BHK-21细胞测定其毒价TCID50为10-8.042/0.1 mL;PCR体外扩增可见其扩增片段与预期目的条带相一致;据此分离病毒株的上述特征,鉴定该病毒为猪伪狂犬病病毒,并命名为SX09。 |
| 关 键 词： | 猪伪狂犬病病毒 分离 鉴定 BHK-21 |
The Separation and Identification of Pig Pseudo Hydrophobic Virus |
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| YANG Qing-fang,NING Guan-bao,LI Jun-da. The Separation and Identification of Pig Pseudo Hydrophobic Virus[J]. Journal of Shanxi Agricultural Sciences, 2011, 39(8): 886-889. DOI: 10.3969/j.issn.1002-2481.2011.08.34 | |
| Authors: | YANG Qing-fang NING Guan-bao LI Jun-da |
| Affiliation: | YANG Qing-fang1,NING Guan-bao2,LI Jun-da3(1.Jinzhong Vocational & Technical College,Jinzhong 030600,China,2.Shanxi Agricultural University,Taigu 030801,3.Wuxiang County Animal Husbandry and Veterinary,Wuxiang 046300,China) |
| Abstract: | We separated a suspected pseudo rabies virus(PRV) strain from some sick pigs' brain tissue in a Shanxi piggery,and identified it by the BHK-21 cell culture,virus toxic titration,neutralization test,and PCR detection method of the virus.The results were that: The separated virus had typical cytopathy in BHK-21 cell culture blind passage after four generations;Using BHK-21 cells determination,the poison price of TCID50 10-8.042/0.1 mL was got;By polymerase chain reaction(PCR) expand in vitro could see its amp... |
| Keywords: | pig pseudo hydrophobic virus separation identification BHK-21 |
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