| 黄花柳基因组微卫星分离及多态性位点检测 |
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| 引用本文： | 邓玉营,徐立安,张博,诸葛强,黄敏仁,王明庥. 黄花柳基因组微卫星分离及多态性位点检测[J]. 分子植物育种, 2008, 6(1): 89-94 |
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| 作者姓名： | 邓玉营 徐立安 张博 诸葛强 黄敏仁 王明庥 |
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| 作者单位： | 常州工程职业技术学院应用化学技术系,常州,213164;南京林业大学林木遗传与基因工程重点实验室,南京,210037 |
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| 基金项目： | 国家自然科学基金 , 常州市武进区科技局科学项目 |
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| 摘 要： | 以生物素标记的(CT)15和(GT)15为探针进行杂交,借助磁珠筛选出含微卫星的Sau3A I酶切片段,构建了黄花柳微卫星富集文库,获得960个阳性克隆.随机挑选360个阳性克隆进行测序,发现含有微卫星的克隆比例达45%,最后获得的44条非同源性克隆中共含有53个微卫星位点,其中(TC/AG)n,(GA/CT)n,(CA/GT)n比例最高,占74%.随机选取其中的18个微卫星位点设计引物,用5个种个体混合DNA组成模板池,检测引物种间的多态性信息,初步筛选出5对引物在柳属种间有很好的通用性.说明微卫星富集法开发SSR标记不失为一种有效的方法.
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| 关 键 词： | 黄花柳 微卫星富集 微卫星多态性 |
Development of Genomic based Microsatellites from Salix caprea and SSR Polymorphic Loci Identification in Salix Species |
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| Deng Yuying,Xu Lian,Zhang Bo,Zhuge Qiang,Huang Minren,Wang Mingxiu. Development of Genomic based Microsatellites from Salix caprea and SSR Polymorphic Loci Identification in Salix Species[J]. Molecular Plant Breeding, 2008, 6(1): 89-94 |
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| Authors: | Deng Yuying Xu Lian Zhang Bo Zhuge Qiang Huang Minren Wang Mingxiu |
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| Affiliation: | Deng Yuying 2 Xu Li'an 1 Zhang Bo 1 Zhuge Qiang 1 Huang Minren 1 Wang Mingxiu 1 1 The Key Laboralory of Forest Genetics , Gene Engineering,Nanjing Foresty University,Nanjing,210037,2 Department of Applied Chemistry Technology,Changzhou Institute of Engineering Technology,Changzhou,213164 |
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| Abstract: | In present research, we constructed the microsatellites-enriched libraries of Salix caprea that contained 960 Sau3A I-digested microsatellites positive clones identified by the approach of streptavidin magnesphere paramagnetic particles and bloted with the oligoprobes of biotinylated (CT)15 and (GT)15. Three hundred and sixty clones were randomly selected to be sequenced, and the results showed that the tested clone containing microsatellite might account for 45% and 44 clones were identified as non-homolog... |
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| Keywords: | Salix caprea MicrosateUites-enriched Microsatellite polymorphic locus |
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