猪水通道蛋白7基因第4内含子多态性分析

本研究旨在探讨国内外猪种水通道蛋白7(aquaporin 7,AQP7)基因多态性,以期为进一步研究猪AQP7基因与脂肪沉积性状的相关性提供理论依据。采用PCR-RFLP方法检测了猪AQP7基因第4内含子G/A在6个猪群中的多态性分布。检测结果表明,经PstⅠ酶切后发现了GG、AG和AA 3种基因型。在莱芜猪、里岔猪、鲁莱猪、杜洛克猪和大约克猪5个猪种中G均为优势等位基因,而在沂蒙猪中A为优势等位基因。χ²检验结果发现,基因型分布在莱芜猪、鲁莱猪和沂蒙猪群体达到了哈代－温伯格平衡(P>0.05),而里岔猪、杜洛克猪和大约克猪群体中均未达到平衡状态(P<0.05);不同基因型在群体间的分布差异极显著(P<0.01)。群体遗传特性分析表明,有效等位基因数在0.3084~0.4953之间,多态信息含量在0.2609~0.3726之间,属于中度多态。本研究结果提示,地方猪种、引进猪种和培育猪种AQP7基因内含子4的基因型分布存在明显差异,A等位基因作为脂肪沉积的有利等位基因尚需进一步验证。

Analysis of the Polymorphisms in Intron 4 of Pig Aquaporin 7 Gene

The aim of this study was to discuss the polymorphisms in intron 4 of AQP7 gene in native and exotic pig breeds,and the results will provide useful scientific data to for further research on correlation between AQP7 gene and fat deposition traits.The distribution of PstⅠ polymorphisms in intron 4 of AQP7 gene were detected by PCR-RFLP among 42 Laiwu pigs,45 Licha pigs,31 Yimeng pig,55 Lulai pigs(Yorkshire × Laiwu),39 Duroc pigs,and 34 Yorkshire.The results indicated the genotypes of the AQP7 PstⅠ were GG,AG and AA from 6 populations.Allele G was dominant in Laiwu pig,Licha pig,Lulai pig,Duroc and Yorkshire,and allele A was dominant in Yimeng pig,respectively.A Chi-square analysis showed that Laiwu pig,Lulai pig,and Yimeng pig populations had reached Hardy-Weinberg equilibrium(P>0.05),and Licha,Duroc and Yorkshire were unequilibrium(P<0.05).Distribution of GG,AG and AA genotypes was significantly(P<0.01) in 6 populations.Population genetic character analysis showed that effective number of alleles ranged from 0.3084 to 0.4953 and polymorphism information content(PIC)were between 0.2609 and 0.3726,being moderate polymorphic locus.The results showed that genotype distribution in AQP7 intron 4 was significantly different between native pig breeds,exotic pig breeds and cultivated pig breed,while allele A was useful allele for fat deposition still need to verify further.