蒙古绵羊胎儿皮肤成纤维细胞分离培养与特性分析

本研究以蒙古绵羊胎儿为材料,进行了胎儿性别鉴定,结果显示为雄性。取其皮肤组织进行离体培养,对所获得的细胞进行纯化,筛选出成纤维细胞。通过形态观察发现,在10代以内,细胞形态良好,随着代数增加梭型细胞有拉长趋势,在第20代时梭形明显拉长,单位面积细胞数目大量减少;同时做了F5、F10代细胞的生长曲线,发现随着代数的增长,细胞生长力略有下降,但趋势不明显;对F5、F10代细胞做了核型分析,二倍体比率分别为85.71%和76.67%,这满足了作为核移植供体细胞的要求;细胞冻存后复苏贴壁情况良好。

Separation Culture and Characterization Analysis of Mongolian Sheep Fetal Skin Fibroblasts

In this study,selecting Mongolian sheep fetus as materials,determining the fetas sex,the results showed that they were male.The skin tissues were cultuered in vitro and the cells obtained were purified and fibroblasts were screened.Through observing,the cell modality was better in 10 generations.The spindle-shape cells showed a elongated trend with the generations increasing and the spindle-shape cells stretched obviously in 20th generation and the number of cells per unit area reduced significantly.In the meantime,the growth force of cell s declined slightly with the increasing generations by the growth curve of F5 and F10 cells.But the trend was not obvious.Through the karyotype analysis of F5 and F10 cells,we found that diploid rates were 85.71% and 76.67%,which met the reques of nuclear transfer somatic cell.Recovery of adherent cells after cryopreservation was in good conditions.