| 禽流感病毒株A/duck/Fujian/31/2007(H5N1)株M1基因的克隆及序列分析 |
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| 引用本文： | 布日额,任晓峰,李明刚,吴金花,孙立杰. 禽流感病毒株A/duck/Fujian/31/2007(H5N1)株M1基因的克隆及序列分析[J]. 中国畜牧兽医, 2012, 39(5): 27-30 |
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| 作者姓名： | 布日额 任晓峰 李明刚 吴金花 孙立杰 |
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| 作者单位： | 1. 内蒙古民族大学生命科学学院, 内蒙古通辽 028043;2. 内蒙古民族大学乳源性致病菌研究所, 内蒙古通辽 028043;3. 东北农业大学动物医学院, 黑龙江哈尔滨 150030;4. 北京庄笛浩禾生物医学科技有限公司, 北京 100043 |
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| 基金项目： | 内蒙古自治区自然科学基金 |
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| 摘 要： | 参照GenBank公布的禽流感病毒基质蛋白M1基因序列,设计合成了1对特异性引物,采用RT-PCR法成功扩增并克隆了禽流感病毒M1基因。序列测定结果为M1基因cDNA全长759 bp,编码252个氨基酸。将其序列与数株甲型流感病毒M1基因序列进行比较,M1基因核苷酸同源性为99.1%~99.6%,推导氨基酸同源性为98.8%~99.6%,生物信息学分析表明,M1基因编码的蛋白质具有亲水性,有很强的抗原性,M1蛋白共有6个潜在的N-糖基化位点,可能存在3个蛋白激酶C磷酸化位点,存在2个酪蛋白激酶Ⅱ磷酸化位点。
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| 关 键 词： | 禽流感病毒 M1基因 克隆 序列分析 |
| 收稿时间： | 2011-11-21 |
Cloning and Sequencing Analysis M1 Gene of Avian Influenza Virus Strain A/duck/Fujian/31/2007 (H5N1) |
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| BU Ri-e , REN Xiao-feng , LI Ming-gang , WU Jin-hua , SUN Li-jie. Cloning and Sequencing Analysis M1 Gene of Avian Influenza Virus Strain A/duck/Fujian/31/2007 (H5N1)[J]. China Animal Husbandry & Veterinary Medicine, 2012, 39(5): 27-30 |
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| Authors: | BU Ri-e REN Xiao-feng LI Ming-gang WU Jin-hua SUN Li-jie |
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| Affiliation: | 1. College of Life Science, Inner Mongolia University for Nationalities, Tongliao 028043, China;2. Research Institute of Pathogenic in Milk, Inner Mongolia University for Nationalities, Tongliao 028043, China;3. Collgege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China;4. Beijing Zhuang Di Hao He Biomedical Technology Co., Ltd., Beijing, 100043, China |
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| Abstract: | A pair of primer was designed according to the sequences of avian influenza virus(AIV) on GenBank.Total RNA from AIV was taken as template to make RT-PCR amplification.The M1 gene of AIV was amplified by RT-PCR.M1 gene was 759 bp in length,encoding 252 amino acids.Comparing with other published M1 cDNA sequences.It showed that the homology of M1 gene was from 99.1% to 99.6%,and the homology of amino acids was from 98.8% to 99.60%.Bioinformatics analysis of this sequence showed that the protein of M1 was hydrophilicand highly antigenic.M1 includes six potential N-glycosylation sites,three potential proteinkinase C phosphorylation sites,two potential caseinkinase Ⅱ phosphorylation sites. |
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| Keywords: | avian influenza virus M1 gene cloning sequence analysis |
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