免疫荧光检测技术及其在寄生虫检测中的应用进展

免疫荧光技术(immunofluorescence technique)是在生物化学、显微镜技术和免疫学基础上发展起来的一项检测技术,是用荧光标记的抗体或抗原与被检样品中相应的抗原或抗体结合,在显微镜下检测荧光,并对样品进行分析的方法。它把显微镜技术的精确性和免疫学检测的特异性、敏感性有机地结合在一起。这一方法的特点是特异性强、灵敏度高。作为一种快速诊断方法,目前广泛应用于病毒、细菌病的诊断,也是许多动物寄生虫病(如弓形虫病)的常规诊断方法。随着标记抗体、抗原的普及,免疫荧光技术在寄生虫病诊断方面的应用将更加广泛。

Brief Introduction of Immunefluorescent Test Technology and Application Progress on Parasites Detection

Immunofluorescence technique is a new detection technology which is based on biochemistry,microscopy and immunology.Its basic principle is using fluorescence-labeled antibody or antigen to combine with the antigen or antibody in the samples,and the fluorescence can be recognized under a microscope.The microscopic accuracy and the immunological specificity and sensitivity can be organically combined in this method.As a rapid diagnostic method,immunofluorescence technique is widely used in medical and biological research.It is a general method in the diagnosis of virus,bacterium or parasite caused diseases,such as Toxoplasma gondii infection.With the development of labeled antigen and antibody,immunofluorescence technique will be widely used in parasitosis diagnosis.