青年母猪不同生殖器官神经营养因子-4/5的表达研究

本研究旨在探讨神经营养因子-4/5(neuroteophin-4/5, NT-4/5)在青年母猪卵泡期与黄体期卵巢、输卵管及子宫3种生殖器官中表达情况。采用实时荧光定量RT-PCR方法与Western blotting技术对青年母猪卵泡期与黄体期卵巢、输卵管及子宫中的NT-4/5 mRNA与蛋白质表达进行检测。结果表明,NT-4/5 mRNA与蛋白质在卵泡期与黄体期卵巢、输卵管及子宫中均有表达,且卵泡期卵巢、子宫NT-4/5 mRNA表达水平均显著高于黄体期(P＜0.05),而卵泡期输卵管NT-4/5 mRNA表达水平显著低于黄体期(P＜0.05)。试验结果为进一步研究NT-4/5参与青年母猪生殖道生理功能的调节奠定基础。

Expression of Neurotrophin-4/5 in the Reproductive Organs of the Prepubertal Gilts

The expression of neurotrophin-4/5(NT-4/5) mRNA and protein in ovary,oviduct and uterus during the follicle and luteal phase of the pubertal gilts were investigated by quantitative real-time PCR and Western blotting.The results showed that the mRNA and protein of NT-4/5 were present in ovary,oviduct and uterus during the follicle and luteal phase.In addition,the expression amount of NT-4/5 mRNA in ovary,uterus during the follicle stage were significantly higher than the luteal phase(P<0.05),however,the expression level of NT-4/5 mRNA in oviduct during the follicle phase was significantly lower than the luteal phase(P<0.05).In collection,these results strongly suggest that local production of the NT-4/5 may particulate in the regulation of reproductive functions in the reproductive functions in the reproductive tract of the prepubertal gilts.